重组Anti-WSX-1抗体[EPR20863-3] - BSA and Azide free (ab273155)
![Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)](https://www.abcam.cn/ps/products/273/ab273155/Images/ab273155-392338-antiwsx1antibodyepr208633westernblotwildtypemousespleen.jpg "Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20863-3] to WSX-1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-WSX-1抗体[EPR20863-3] - BSA and Azide free
参阅全部 WSX-1 一抗 -
描述
兔单克隆抗体[EPR20863-3] to WSX-1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type mouse spleen and bone marrow tissue lysates.
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常规说明
ab273155 is the carrier-free version of ab220359.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20863-3 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 110, 120 kDa (predicted molecular weight: 69 kDa).
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 110, 120 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms. -
组织特异性
Highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. Weakly expressed in other tissues examined including heart, brain, fetal and adult lung, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine, kidney and colon. In the lymphoid system, higher level expression in CD4+ T-cell subsets than in CD8+ T-cell subsets. Also weaker expression in CD19+ B-cells and monocytes. -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 3 fibronectin type-III domains. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 50931 Mouse
- SwissProt: O70394 Mouse
- Unigene: 38386 Mouse
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别名
- Class I cytokine receptor antibody
- CRL1 antibody
- Cytokine receptor-like 1 antibody
see all
图片
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Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)All lanes : Anti-WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution
Lane 1 : Wild-type mouse spleen (1) tissue lysate
Lane 2 : Wild-type mouse spleen (2) tissue lysate
Lane 3 : Wild-type mouse spleen (3) tissue lysate
Lane 4 : IL27Ra knockout mouse spleen (1) tissue lysate
Lane 5 : IL27Ra knockout mouse spleen (2) tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 110-120 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data from collaborator.
Exposure time: 1 minute.
WSX-1 = IL27Ra
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (ab220359).
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![Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)](https://www.abcam.cn/ps/products/273/ab273155/Images/ab273155-2-benefits-of-recombinant-antibodies.png)
Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab273155 被引用在 1 文献中.
- He H et al. Aging-induced IL27Ra Signaling Impairs Hematopoietic Stem Cells. Blood N/A:N/A (2020). PubMed: 32305041
