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重组RabMAb

重组Anti-Wnt7a抗体[EPR23471-125] - BSA and Azide free (ab274324)

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Western blot - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
  • Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23471-125] to Wnt7a - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Wnt7a抗体[EPR23471-125] - BSA and Azide free
    参阅全部 Wnt7a 一抗

  • 描述

    兔单克隆抗体[EPR23471-125] to Wnt7a - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HaCaT and BxPC-3 whole cell lysates; Mouse P7 hippocampus tissue lysate. ICC/IF: HaCaT cells.

  • 常规说明

    ab274324 is the carrier-free version of ab274321.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274324于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 40, 42 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 40, 42 kDa (predicted molecular weight: 39 kDa).
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.

靶标

  • 功能

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.

  • 组织特异性

    Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.

  • 疾病相关

    Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
    Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia.

  • 序列相似性

    Belongs to the Wnt family.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Protein Wnt-7a antibody
      • Protein Wnt-7a precursor antibody
      • Proto oncogene Wnt7a protein antibody
      • proto-oncogene wnt7a protein antibody
      • wingless-type MMTV integration site family, member 7A antibody
      • Wnt family member 7A antibody
      • WNT7A antibody
      • WNT7A_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
      Western blot - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
      All lanes : Anti-Wnt7a antibody [EPR23471-125] (ab274321) at 1/1000 dilution

      Lane 1 : HaCaT (human skin keratinocyte) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : Mouse P7 hippocampus tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
      Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 40-42 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile observed is consistent with what has been described in the literature (PMID: 25978974, 30143647, 30770219). 

      Negative control: HeLa cells (PMID: 25978974).

      This blot was developed using a higher sensitivity ECL substrate.

      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321)

    • Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
      Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HaCaT and HeLa cells labelling Wnt7a with ab274321 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in HaCaT cells. Low expression cell line: HeLa (PMID: 25978974). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000  dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321).

    • Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
      Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab274324？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab274324 尚未被引用在任何文献中。

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