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AB274324

重组Anti-Wnt7a抗体[EPR23471-125] - BSA and Azide free

Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free

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Rabbit Recombinant Monoclonal WNT7A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse samples.

查看别名

Protein Wnt-7a, WNT7A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (AB274324)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (AB274324)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HaCaT and HeLa cells labelling Wnt7a with ab274321 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in HaCaT cells. Low expression cell line : HeLa (PMID : 25978974). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321).

Western blot - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (AB274324)
  • WB

Lab

Western blot - Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (AB274324)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 25978974, 30143647, 30770219).

Negative control : HeLa cells (PMID : 25978974).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321)

All lanes:

Western blot - Anti-Wnt7a antibody [EPR23471-125] (<a href='/products/primary-antibodies/wnt7a-antibody-epr23471-125-ab274321'>ab274321</a>) at 1/1000 dilution

Lane 1:

HaCaT (human skin keratinocyte) whole cell lysate at 40 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 4:

Mouse P7 hippocampus tissue lysate at 40 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 40-42 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23471-125

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

ab274324 is the carrier-free version of ab274321.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.
Biological function summary

This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.

Pathways

Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.

Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed : 16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
See full target information WNT7A
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搭配使用产品

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

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