AB227229

Anti-Wnt5a抗体

Name: Wnt5a抗体 (ab227229)| Abcam中文官网
Brand: Abcam Limited
SKU: AB227229
Price: 4748 CNY
Availability: InStock

Anti-Wnt5a antibody

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(7 Publications)

Rabbit Polyclonal WNT5A antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT5A.

查看别名

Protein Wnt-5a, WNT5A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

Immunohistochemical analysis of paraffin-embedded rat heart tissue stained for secreted Wnt5a protein with ab227229 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Supplier Data

Western blot - Anti-Wnt5a antibody (AB227229)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Wnt5a antibody (ab227229) at 1/500 dilution

All lanes:

Rat heart extract at 50 µg

Predicted band size: 42 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human WNT5A. The exact immunogen used to generate this antibody is proprietary information.

P41221

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.

Biological function summary

Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.

Pathways

Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.

Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

See full target information WNT5A

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1534118 PubMed40365308

2025

Triptolide alleviates psoriasis through inhibiting the Wnt5a/β-Catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Eryang Chen,Lei Wang,Qu Wang,Yan Cai,Yaning Dou,Hongyan Qu,Junyi Zhu,Haiyang Zhao,Suqing Zheng,Chengguang Zhao,Bin Chen

Journal of orthopaedic translation 50:144-157 PubMed40171108

2025

Moderate static magnetic field regulates iron metabolism and salvage bone loss caused by iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxiao Zhen,Shenghang Wang,Jiancheng Yang,Gejing Zhang,Chao Cai,Jianping Wang,Aifei Wang,Youjia Xu,Yanwen Fang,Min Wei,Dachuan Yin,Xinle Luo,Ming Gong,Hao Zhang,Peng Shang

Iranian journal of basic medical sciences 26:812-819 PubMed37396938

2023

MiR26-5p inhibits pathological pulmonary microvascular angiogenesis via down-regulating WNT5A.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Feng Gao,Dan Li,Jinquan Wang

Open life sciences 17:1591-1599 PubMed36518887

2022

PHF8 promotes osteogenic differentiation of BMSCs in old rat with osteoporosis by regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Kai Huang,Hongbin Dai,Chunhe Sha

Molecular cell 82:4018-4032.e9 PubMed36332605

2022

LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Dongpeng Wang,Yajing Hao,Shuheng Wu,Jianjun Luo,Yuanchao Xue,Di Wang,Guohong Li,Lihui Liu,Changwei Shao,Huiyan Li,Jinfeng Yuan,Maoxiang Zhu,Xiang-Dong Fu,Xiao Yang,Runsheng Chen,Yan Teng

International journal of experimental pathology : PubMed33350543

2020

Circular RNA ITCH mediates HO-induced myocardial cell apoptosis by targeting miR-17-5p via wnt/β-catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nengfeng Zhang,Xu Wang

Gene 761:145038 PubMed32777532

2020

Downregulated circular RNA zRANB1 mediates Wnt5a/β-Catenin signaling to promote neuropathic pain via miR-24-3p/LPAR3 axis in CCI rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wei,Lin Li,Yang Zhang,Min Zhang,Zhen Su

View all publications

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Anti-Wnt5a抗体

Anti-Wnt5a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

Western blot - Anti-Wnt5a antibody (AB227229)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (7)

Abcam Product Promise

Anti-Wnt5a抗体

Anti-Wnt5a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

Western blot - Anti-Wnt5a antibody (AB227229)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (7)

Abcam Product Promise