AB219412

Anti-Wnt3a 抗体 [EPR21889]

Anti-Wnt3a antibody [EPR21889]

RabMAb
Recombinant
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(96 Publications)

Anti-Wnt3a antibody [EPR21889] (ab219412) is a rabbit monoclonal antibody detecting Wnt3a in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

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Protein Wnt-3a, WNT3A

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of rat E14.5 (PMID : 15509764; PMID : 16410414) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of mouse E14.5 (PMID : 15509764; PMID : 16410414) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] (AB219412)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] (AB219412)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cells labeling Wnt3a with ab219412 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in L Wnt-3A cell line. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only.

Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] (AB219412)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] (AB219412)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cell line (right)labeling Wnt3a with ab219412 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Two Wnt3a-expressing populations can be seen labeled in this stably transfected cell line.

Supplier Data

Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (AB219412)

Wnt3a was immunoprecipitated from 0.35 mg L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate with ab219412 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1 : L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate 10 μg (Input).
Lane 2 : ab219412 IP in L Wnt-3A whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219412 in L Wnt-3a whole cell lysate.
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (ab219412)

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Wnt3a antibody [EPR21889] (AB219412)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal skin lysate at 10 µg

Lane 3:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Wnt3a antibody [EPR21889] (AB219412)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/20000 dilution

Lane 1:

His-tagged human Wnt3 recombinant protein (aa22-355), 40 ng

Lane 2:

His-tagged human Wnt3a recombinant protein (aa19-352), 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Wnt3a antibody [EPR21889] (AB219412)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 minutes; Lane 2 : 3 seconds.

All lanes:

Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/1000 dilution

Lane 1:

L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) supernatant at 20 µL

Lane 2:

L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

false

不同偶联物与剂型 (10)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Wnt3a antibody [EPR21889]
Carrier free

Anti-Wnt3a antibody [EPR21889] - BSA and Azide free
578 PE

PE Anti-Wnt3a antibody [EPR21889]
660 APC

APC Anti-Wnt3a antibody [EPR21889]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Wnt3a antibody [EPR21889]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Wnt3a antibody [EPR21889]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Wnt3a antibody [EPR21889]
HRP

HRP Anti-Wnt3a antibody [EPR21889]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Wnt3a antibody [EPR21889]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Wnt3a antibody [EPR21889]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21889

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Unsuitable for human IHC-P. Rat species is recommended based on IHC result. We do not guarantee western blot for rat.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

What is this antibody validated in?
Anti-Wnt3a antibody [EPR21889] (ab219412) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Wnt3a?
Anti-Wnt3a [EPR21889] (ab219412) specifically detects a band for Wnt3a (UniProt: P56704) at a molecular weight of 39kDa.

Trusted by the scientific community
Anti-Wnt3a [EPR21889] (ab219412) was first used in a scientific publication in 2018 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21889] also available for your convenience: ab219412, Carrier free - ab234099, Alexa Fluor® 647 - ab270093, PE - ab305728, APC - ab305729, HRP - ab305730, Alkaline Phosphatase - ab308719, Alexa Fluor® 488 - ab309684, Alexa Fluor® 594 - ab310437, Alexa Fluor® 555 - ab311964, Alexa Fluor® 568 - ab312436, Alexa Fluor® 750 - ab321404

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/Β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing Β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3Β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like Β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

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文献 (96)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40709387

2025

Bmi1 regulates neural differentiation of mesenchymal stem cells through the Wnt3a‑RhoA signaling pathway to repair ischemic brain injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kunling Chen,Hongjie Zhou,Jie Zhang,Yiwei Zhang,Xiaobing Dou,Qin Yu,Liping Zhou

BMC biotechnology 25:67 PubMed40624715

2025

CDCP1 promotes the malignant phenotypes of nasopharyngeal carcinoma via the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoliang Bie,Shuang Cheng,Weiping Huang,Zhongpu Yin,Jianzhi Liu

Nature communications 16:4124 PubMed40319020

2025

Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Jun-Kit Hu,Xiaoyu Tracy Cai,Jesse Simons,Jina Yun,Justin Elstrott,Heinrich Jasper

Journal of molecular histology 56:144 PubMed40285932

2025

Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Jun Li,Haoyu Wang,Xu Chang,Qinglong Kong

Cellular and molecular life sciences : CMLS 82:165 PubMed40249512

2025

Involvement of miRNA-204 carried by the exosomes of macrophages in the AT2 receptor-mediated improvement of vascular calcification.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yu Bai,Xiao-Rui Lv,Hai-Bo Gu,Hui Li,Bao-Shuai Shan

Molecular neurobiology 62:8279-8297 PubMed39992588

2025

Identification and Validation of Biomarkers for Alzheimer's Disease Based on Akt and Wnt Signaling Pathways in Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Han Wang,Hong-Yun Wu,Chao Xin,Kai-Xin Zhang,Ji-Wei Zhang,Hong-Wei Zhi

Biomolecules & biomedicine 25:2035-2049 PubMed39976471

2025

Study on the mechanism of Wnt/β-catenin pathway mediated by pterostilbene to reduce cerebral ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jin,Chunwang Fu,Ming Guo,Qiang Yang

Bone research 13:17 PubMed39865089

2025

Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Alhashmi,Abdulrahman M E Gremida,Santosh K Maharana,Marco Antonaci,Amy Kerr,Shijian Fu,Sharna Lunn,David A Turner,Noor A Al-Maslamani,Ke Liu,Maria M Meschis,Hazel Sutherland,Peter Wilson,Peter Clegg,Grant N Wheeler,Robert J van 't Hof,George Bou-Gharios,Kazuhiro Yamamoto

Open medicine (Warsaw, Poland) 20:20241119 PubMed39822992

2025

Knockdown of CCNB1 alleviates high glucose-triggered trophoblast dysfunction during gestational diabetes via Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Biru Xiao,Wenmiao Zhang,Nini Ji,Qiuyue Chen

Cell biology and toxicology 41:5 PubMed39707064

2024

Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.

Applications