AB172612

重组Anti-Wnt3 and Wnt3a抗体[EPR4920(2)]

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)]

RabMAb
Recombinant
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(27 Publications)

Rabbit Recombinant Monoclonal WNT3A antibody. Suitable for WB and reacts with Transfected cell lysate - Human samples. Cited in 27 publications.

查看别名

Protein Wnt-3a, WNT3A

1 Images

Supplier Data

Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (AB172612)

All lanes:

Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612) at 1/10000 dilution

Lane 1:

CHO cell lysate, non-transfected at 10 µg

Lane 2:

CHO cell lysate, transfected with Human Wnt3a at 10 µg

Predicted band size: 39 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4920(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

This antibody was developed as part of a collaboration with Karl Willert's lab from the University of California at San Diego.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Wnt3 and Wnt3a both members of the Wnt family are secreted glycoproteins involved in several signaling pathways. Commonly known as Wnt3 and Wnt3a for simplicity they have an approximate mass of around 40 kDa. These proteins exhibit expression during development stages and in various adult tissues. They function mechanistically by binding to Frizzled family receptors activating downstream signaling cascades. Wnt3 is predominantly expressed in the central nervous system while Wnt3a shows wide expression across mesoderm and ectoderm tissues.

Biological function summary

Wnt3 and Wnt3a are key players in cell proliferation differentiation and migration. These proteins are part of the Wnt signaling complex which influences gene expression important for embryonic development and tissue homeostasis. The Wnt3 and Wnt3a promote stem cell maintenance and expansion by regulating transcriptional activators and repressors. Their role extends to maintaining cellular architecture through interaction with cytoskeletal elements.

Pathways

Wnt3 and Wnt3a are integral components of the canonical Wnt/β-catenin pathway and the Wnt/Ca2+ non-canonical pathway. Through these pathways they impact various biological processes essential to cellular growth and differentiation. Wnt3a often interacts with β-catenin a well-known mediator that influences gene expression by entering the nucleus. Both Wnts regulate pathways that include Dishevelled (Dvl) proteins highlighting their interconnectedness in cell signaling networks.

Alterations in Wnt3 and Wnt3a signaling contribute to cancer and neurodegenerative diseases. Abnormal Wnt/β-catenin signaling links to colorectal cancer where excessive β-catenin activity supports tumor growth. Moreover disrupted Wnt signaling can impact Alzheimer's disease pathogenesis through interactions with amyloid precursor protein and tau. These connections highlight how Wnt3 and Wnt3a influence disease states impacting therapeutic strategies targeting Wnt pathway components.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

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文献 (27)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70499 PubMed40099939

2025

Circular RNA circHSPA8 Aggravates Metastasis by Acting as a Competitive Inhibitor of miR-195-5p to Upregulate WNT3A Expression in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoying Han,Xiaojuan Yu,Chenlong Wang,Xiaoyu Song,Xiaomin Zhong,Renhua Guo,Weiyong Yu,Chao Luo

Gut microbes 16:2347722 PubMed38706205

2024

Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Wei Xie,Shang Cai,Hai-Yan Lu,Feng-Ling Tang,Rui-Qiu Zhu,Ye Tian,Ming Li

Cell cycle (Georgetown, Tex.) 22:1544-1562 PubMed37287276

2023

Exosomal miR-18a-5p promotes EMT and metastasis of NPC cells via targeting BTG3 and activating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhong,Qihong Nie,Renrui Wu,Yun Huang

Journal of gastroenterology 58:444-457 PubMed36739585

2023

LAT1 expression influences Paneth cell number and tumor development in Apc mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Sui,Namiko Hoshi,Ryuichi Ohgaki,Lingling Kong,Ryutaro Yoshida,Norihiro Okamoto,Masato Kinoshita,Haruka Miyazaki,Yuna Ku,Eri Tokunaga,Yuki Ito,Daisuke Watanabe,Makoto Ooi,Masakazu Shinohara,Kengo Sasaki,Yoh Zen,Takenori Kotani,Takashi Matozaki,Zibin Tian,Yoshikatsu Kanai,Yuzo Kodama

Cell reports 40:111293 PubMed36044854

2022

RBM45 is an mA-binding protein that affects neuronal differentiation and the splicing of a subset of mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Seung H Choi,Mathieu N Flamand,Bei Liu,Huanyu Zhu,Meghan Hu,Melanie Wang,Jonathon Sewell,Christopher L Holley,Hashim M Al-Hashimi,Kate D Meyer

Environmental toxicology 37:2540-2551 PubMed35841383

2022

LOXL3-promoted hepatocellular carcinoma progression via promotion of Snail1/USP4-mediated epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Runze Shang,Shunle Li,Yifan Ren,Lin Shen,Longbao Yang,Shuo Chen,Xi Chen,Junhui Li,Meng Xu

ESC heart failure 8:3935-3946 PubMed34165260

2021

Telocytes-derived extracellular vesicles alleviate aortic valve calcification by carrying miR-30b.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Yang,Yihu Tang,Xiaowen Chen,Yang Yang

Aging 13:262-278 PubMed33436530

2021

Combined transplantation of neural stem cells and bone marrow mesenchymal stem cells promotes neuronal cell survival to alleviate brain damage after cardiac arrest microRNA-133b incorporated in extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Li,Jie Zhang,Anbao Chen,Rui Liao,Yongchun Duan,Yuwei Xu,Lili Tao

Journal of cellular and molecular medicine 25:1958-1971 PubMed33434361

2021

FoxM1 promotes Wnt/β-catenin pathway activation and renal fibrosis via transcriptionally regulating multi-Wnts expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Xie,Naijun Miao,Dan Xu,Zhuanli Zhou,Jiayun Ni,Fan Yin,Yanzhe Wang,Qian Cheng,Panpan Chen,Jingyao Li,Peiqing Zheng,Li Zhou,Jun Liu,Wei Zhang,Xiaoxia Wang,Limin Lu

Evidence-based complementary and alternative medic 2020:3025946 PubMed33082821

2020

Fortified S-Allyl L-Cysteine: Animal Safety, Effect on Retinal Ischemia, and Role of Wnt in the Underlying Therapeutic Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Windsor Wen-Jin Chao,Yu-Kuang Chen,Howard Wen-Haur Chao,Wynn Hwai-Tzong Pan,Hsiao-Ming Chao

View all publications

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重组Anti-Wnt3 and Wnt3a抗体[EPR4920(2)]