重组Anti-Wnt16抗体[EPR6064] (ab109437)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6064] to Wnt16
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Wnt16抗体[EPR6064]
参阅全部 Wnt16 一抗 -
描述
兔单克隆抗体[EPR6064] to Wnt16 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji, LnCaP, HepG2, 293T, Mouse brain, and Rat brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 6.05 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6064 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109437于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
靶标
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功能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
组织特异性
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas. -
序列相似性
Belongs to the Wnt family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 51384 Human
- Entrez Gene: 93735 Mouse
- Entrez Gene: 500047 Rat
- Omim: 606267 Human
- SwissProt: Q9UBV4 Human
- SwissProt: Q9QYS1 Mouse
- Unigene: 272375 Human
- Unigene: 137403 Mouse
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别名
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
see all
图片
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All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab109437 被引用在 10 文献中.
- Shi J et al. Matrine improves the hepatic microenvironment and reverses epithelial-mesenchymal transition in hepatocellular carcinoma by inhibiting the Wnt-1/β-catenin signaling pathway. Am J Transl Res 15:5047-5070 (2023). PubMed: 37692966
- Qi J et al. Circ-Ctnnb1 Regulates Neuronal Injury in Spinal Cord Injury through the Wnt/β-Catenin Signaling Pathway. Dev Neurosci 44:131-141 (2022). PubMed: 34929706
- Ye W et al. USF3 modulates osteoporosis risk by targeting WNT16, RANKL, RUNX2, and two GWAS lead SNPs rs2908007 and rs4531631. Hum Mutat 42:37-49 (2021). PubMed: 33058301
- Guo G et al. Hsa_circ_0000479 as a Novel Diagnostic Biomarker of Systemic Lupus Erythematosus. Front Immunol 10:2281 (2019). PubMed: 31608065
- Ding SM et al. The role of cancer-associated fibroblast MRC-5 in pancreatic cancer. J Cancer 9:614-628 (2018). WB ; Human . PubMed: 29483967
- Hildebrandt S et al. Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo. Sci Rep 8:8711 (2018). WB ; Mouse . PubMed: 29880826
- Spinelli C et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643
- Munguía-Reyes A et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Andres-Mateos E et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other species . PubMed: 23155423