Anti-Wnt11抗体(ab96730)
Key features and details
- Rabbit polyclonal to Wnt11
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Wnt11抗体
参阅全部 Wnt11 一抗 -
描述
兔多克隆抗体to Wnt11 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).
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阳性对照
- WB: HEK-293T, A431, COLO 205 cell lysate, mouse heart white adipose tissue extract. IHC-P: Lung SCC xenograft and mouse colon tissue. IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96730于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
靶标
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功能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
组织特异性
Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas. -
序列相似性
Belongs to the Wnt family. -
翻译后修饰
Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 7481 Human
- Entrez Gene: 22411 Mouse
- Omim: 603699 Human
- SwissProt: O96014 Human
- SwissProt: P48615 Mouse
- Unigene: 108219 Human
- Unigene: 22182 Mouse
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别名
- HWNT11 antibody
- Protein Wnt-11 antibody
- Wingless-related MMTV integration site 11 antibody
see all
图片
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Western blot - Anti-Wnt11 antibody (ab96730)All lanes : Anti-Wnt11 antibody (ab96730) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)Paraffin-embedded mouse colon tissue stained Wnt11 using ab96730 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) using ab96730 at 1/200 dilution. Lower image merged with DNA probe.
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Western blot - Anti-Wnt11 antibody (ab96730)Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse heart tissue extractc at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt11 antibody (ab96730)Paraffin-embedded lung SCC xenograft satined Wnt11 using ab96730 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt11 antibody (ab96730)Immunofluorescence analysis of ice-cold MeOH-fixed (5 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Wnt11 (green) at cytoplasm using ab96730 at 1/500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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Western blot - Anti-Wnt11 antibody (ab96730)Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + Mouse white adipose tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
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Western blot - Anti-Wnt11 antibody (ab96730)Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + COLO 205 (human colon adenocarcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa10% SDS-PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96730 被引用在 4 文献中.
- Li C et al. Comprehensive analysis of pathological changes in hip joint capsule of patients with developmental dysplasia of the hip. Bone Joint Res 10:558-570 (2021). PubMed: 34465146
- Roettger A et al. Inhibitory Effect of 1,8-Cineol on ß-Catenin Regulation, WNT11 Expression, and Cellular Progression in HNSCC. Front Oncol 7:92 (2017). PubMed: 28589081
- Keats EC et al. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells 32:1649-60 (2014). PubMed: 24496952
- Matise LA et al. Lack of transforming growth factor-ß signaling promotes collective cancer cell invasion through tumor-stromal crosstalk. Breast Cancer Res 14:R98 (2012). PubMed: 22748014