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Anti-WNK4抗体(ab126477)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK4 antibody (ab126477)

    Key features and details

    • Rabbit polyclonal to WNK4
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-WNK4抗体

    • 描述

      兔多克隆抗体to WNK4

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      antigen sequence corresponding to amino acids 1005-1138 of Human WNK4.

    • 阳性对照

      • Human kidney tissue.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab126477于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.

    • 组织特异性

      Expressed in kidney, colon and skin.

    • 疾病相关

      Pseudohypoaldosteronism 2B

    • 序列相似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Phosphorylated by WNK1 and WNK3.
      Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation and increased expression of KCNJ1 at the cell surface. Ubiquitinated by the BCR(KLHL2) complex.

    • 细胞定位

      Cell junction, tight junction. Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).
    • Information by UniProt

    • 数据库链接

      • 别名

        • PHA 2B antibody
        • PHA2B antibody
        • PRKWNK 4 antibody
        • PRKWNK4 antibody
        • Protein kinase lysine deficient 4 antibody
        • Protein kinase lysine-deficient 4 antibody
        • Protein kinase with no lysine 4 antibody
        • Serine/threonine protein kinase WNK4 antibody
        • Serine/threonine-protein kinase WNK4 antibody
        • WNK lysine deficient protein kinase 4 antibody
        • Wnk4 antibody
        • WNK4_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK4 antibody (ab126477)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK4 antibody (ab126477)
        ab126477, at 1/20 dilution, staining WNK4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      文献 (2)

      发表研究结果有使用 ab126477？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab126477 被引用在 2 文献中.

      • Lashhab R  et al. Expression of the kidney anion exchanger 1 affects WNK4 and SPAK phosphorylation and results in claudin-4 phosphorylation. Heliyon 9:e22280 (2023). PubMed: 38034706
      • Wolley MJ  et al. In Primary Aldosteronism, Mineralocorticoids Influence Exosomal Sodium-Chloride Cotransporter Abundance. J Am Soc Nephrol 28:56-63 (2017). Human . PubMed: 27381844

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      1-2 of 2 Abreviews or Q&A

      Question

      Testing discount code to test ab126477 in rat tissue.

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      Answer

      Thank you for calling Abcam earlier.

      The testing discount details for ab126477 are below along with the new lab discount:

      https://www.abcam.com/index.html?pageconfig=resource&rid=14561

      TESTING DISCOUNT

      DISCOUNT CODE: ******
      Expiration date: **********

      I am very pleased to hear you would like to accept our offer and test ab126477 in rat tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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      Question

      Searching for replacement for ab3965. Needs to work on mouse in WB.

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      Answer

      Thank you very much for your interest in ab126477.

      To our knowledge, ab126477 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you buy ab126477 now, test it in mouse tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab126477 in mouse tissue. I will then send a discount code. This code must be issued before purchasing ab126477 so please wait for my reply before ordering.

      2. Purchase ab126477 either by phone, fax, or online (www.abcam.com).

      3. Test it in mouse tissue.
      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab126477 turns out to be unsuitable for mouse tissue, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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