Anti-WNK1 (phospho T58)抗体(ab53137)
Key features and details
- Rabbit polyclonal to WNK1 (phospho T58)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WNK1 (phospho T58)抗体
参阅全部 WNK1 一抗 -
描述
兔多克隆抗体to WNK1 (phospho T58) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human WNK1 aa 1-100 (phospho T58).
Database link: Q9H4A3 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53137于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/300 - 1/1000. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/300 - 1/1000. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. -
组织特异性
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron. -
疾病相关
Pseudohypoaldosteronism 2C
Neuropathy, hereditary sensory and autonomic, 2A -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
O-glycosylated.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 65125 Human
- Omim: 605232 Human
- SwissProt: Q9H4A3 Human
- Unigene: 744906 Human
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别名
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
- HSN2 antibody
see all
图片
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Western blot - Anti-WNK1 (phospho T58) antibody (ab53137)All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution
Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 251 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WNK1 (phospho T58) antibody (ab53137)ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab53137 被引用在 2 文献中.
- Marshall WS et al. WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function. Biol Open 6:956-966 (2017). PubMed: 28522431
- Cheng CJ & Huang CL Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1. J Am Soc Nephrol 22:460-71 (2011). WB ; Human . PubMed: 21355052