Anti-WISP1抗体- C-terminal (ab178547)
Key features and details
- Rabbit polyclonal to WISP1 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-WISP1抗体- C-terminal
参阅全部 WISP1 一抗 -
描述
兔多克隆抗体to WISP1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human WISP1 aa 354-367 (C terminal). Differs from the related mouse and rat sequences by one amino acid.
Sequence:ADLESYPDFSEIAN
Database link: O95388 -
阳性对照
- WB: Rat heart, kidney and lung tissue lysates; A549, A431 and COLO320 cell lysates. IHC-P: Rat kidney and pancreas tissue; Human placenta tissue ICC: MCF7 cells. IHC-Fr: Rat cardiac muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178547于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 40 kDa.
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说明 |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 40 kDa. |
靶标
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相关性
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 8840 Human
- Entrez Gene: 65154 Rat
- Omim: 603398 Human
- SwissProt: O95388 Human
- SwissProt: Q99PP0 Rat
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别名
- CCN 4 antibody
- CCN family member 4 antibody
- CCN4 antibody
see all
图片
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WISP1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
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WISP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
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All lanes : Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/ml
Lane 1 : rat kidney tissue lysates
Lane 2 : mouse kidney tissue lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/5000 dilution
Predicted band size: 40 kDaElectrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab178547 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.
实验方案
数据表及文件
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Datasheet download
文献 (14)
ab178547 被引用在 14 文献中.
- Chang KS et al. WNT1 Inducible Signaling Pathway Protein 1 Is a Stroma-Specific Secreting Protein Inducing a Fibroblast Contraction and Carcinoma Cell Growth in the Human Prostate. Int J Mol Sci 23:N/A (2022). PubMed: 36232736
- Peng M et al. Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway. Int J Endocrinol 2022:3919161 (2022). PubMed: 36237833
- He W et al. Activation of Frizzled-7 attenuates blood-brain barrier disruption through Dvl/β-catenin/WISP1 signaling pathway after intracerebral hemorrhage in mice. Fluids Barriers CNS 18:44 (2021). PubMed: 34565396
- Yu Z et al. WISP1 and TLR4 on Macrophages Contribute to Ventilator-Induced Lung Injury. Inflammation 43:425-432 (2020). PubMed: 32130574
- Schmoll D et al. Activation of Adenosine Monophosphate-Activated Protein Kinase Reduces the Onset of Diet-Induced Hepatocellular Carcinoma in Mice. Hepatol Commun 4:1056-1072 (2020). PubMed: 32626837