Anti-WISP1 抗体 - C-terminal

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling WISP1 with ab178547 at 1 μg/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

WISP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• ICC

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Immunocytochemistry - Anti-WISP1 antibody - C-terminal (AB178547)

Immunocytochemical analysis of MCF7 cells labeling WISP1 with ab178547 at 1 μg/ml.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

WISP1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab178547 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (AB178547)

Immunohistochemical analysis of frozen rat cardiac muscle sections labeling WISP1 with ab178547 at 1μg/mL (incubated overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling WISP1 with ab178547 at 1 μg/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (AB178547)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling WISP1 with ab178547 at 1 μg/ml.

• WB

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Western blot - Anti-WISP1 antibody - C-terminal (AB178547)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab178547 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.

All lanes:

Western blot - Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/mL

Lane 1:

rat kidney tissue lysates at 30 µg

Lane 2:

mouse kidney tissue lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP at 1/5000 dilution

true

• WB

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Western blot - Anti-WISP1 antibody - C-terminal (AB178547)

All lanes:

Western blot - Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/mL

Lane 1:

Rat heart tissue lysate at 50 µg

Lane 2:

Rat kidney tissue lysate at 50 µg

Lane 3:

Rat lung tissue lysate at 50 µg

Lane 4:

A549 whole cell lysate at 40 µg

Lane 5:

A431 whole cell lysate at 40 µg

Lane 6:

COLO320 whole cell lysate at 40 µg

Predicted band size: 40 kDa

Observed band size: 40 kDa

false