重组Anti-WIPI1抗体[EPR8110] (ab128901)
![Western blot - Anti-WIPI1 antibody [EPR8110] (ab128901)](https://www.abcam.cn/ps/products/128/ab128901/Images/ab128901-142712-anti-wipi1-antibody-epr8110-western-blot.jpg "Western blot - Anti-WIPI1 antibody [EPR8110] (ab128901)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8110] to WIPI1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-WIPI1抗体[EPR8110]
参阅全部 WIPI1 一抗 -
描述
兔单克隆抗体[EPR8110] to WIPI1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human WIPI1 aa 50-150. The exact sequence is proprietary.
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阳性对照
- A375, BxPC3, 293T, C6, RAW264.7, NIH 3T3, and JAR cell lysates; Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.14 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8110 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128901于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
May play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. -
组织特异性
Ubiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues. -
序列相似性
Belongs to the WD repeat SVP1 family.
Contains 3 WD repeats. -
细胞定位
Golgi apparatus > trans-Golgi network. Endosome. Cytoplasmic vesicle > clathrin-coated vesicle. Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. - Information by UniProt
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数据库链接
- Entrez Gene: 55062 Human
- Entrez Gene: 52639 Mouse
- Entrez Gene: 303630 Rat
- Omim: 609224 Human
- SwissProt: Q5MNZ9 Human
- SwissProt: Q8R3E3 Mouse
- Unigene: 463964 Human
- Unigene: 35817 Mouse
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别名
- ATG 18 antibody
- ATG18 antibody
- Atg18 protein homolog antibody
see all
图片
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Western blot - Anti-WIPI1 antibody [EPR8110] (ab128901)All lanes : Anti-WIPI1 antibody [EPR8110] (ab128901) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lane 7 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI1 antibody [EPR8110] (ab128901)](https://www.abcam.cn/ps/products/128/ab128901/Images/ab128901-142711-anti-wipi1-antibody-epr8110-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI1 antibody [EPR8110] (ab128901)ab128901 at 1/50 dilution staining WIPI1 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] (ab128901)](https://www.abcam.cn/ps/products/128/ab128901/Images/ab128901-142710-anti-wipi1-antibody-epr8110.jpg)
OI-RD Scanning - Anti-WIPI1 antibody [EPR8110] (ab128901) -
![Anti-WIPI1 antibody [EPR8110] (ab128901)](https://www.abcam.cn/ps/products/128/ab128901/Images/ab128901-4-benefits-of-recombinant-antibodies.png)
Anti-WIPI1 antibody [EPR8110] (ab128901)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab128901 被引用在 7 文献中.
- Wan W et al. STING directly recruits WIPI2 for autophagosome formation during STING-induced autophagy. EMBO J 42:e112387 (2023). PubMed: 36872914
- Gao M et al. DAP1 regulates osteoblast autophagy via the ATG16L1-LC3 axis in Graves' disease-induced osteoporosis. J Orthop Surg Res 18:711 (2023). PubMed: 37735431
- Liu W et al. Pyroptosis inhibition improves the symptom of acute myocardial infarction. Cell Death Dis 12:852 (2021). PubMed: 34531373
- Sharoar MG et al. Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Mol Psychiatry N/A:N/A (2019). PubMed: 30899091
- Chen K et al. Optineurin-mediated mitophagy protects renal tubular epithelial cells against accelerated senescence in diabetic nephropathy. Cell Death Dis 9:105 (2018). IF . PubMed: 29367621
- Chen K et al. Optineurin inhibits NLRP3 inflammasome activation by enhancing mitophagy of renal tubular cells in diabetic nephropathy. FASEB J N/A:fj201801749RRR (2018). PubMed: 30571313
- Martinez-Lopez N et al. Autophagy proteins regulate ERK phosphorylation. Nat Commun 4:2799 (2013). Mouse . PubMed: 24240988