AB180840

Anti-Wilms Tumor蛋白抗体

Name: Wilms Tumor Protein抗体 (ab180840)| Abcam中文官网
Brand: Abcam Limited
SKU: AB180840
Price: 4922 CNY
Availability: InStock

Anti-Wilms Tumor Protein antibody

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(14 Publications)

Rabbit Polyclonal Wilms Tumor Protein antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WT1.

查看别名

Wilms tumor protein, WT33, WT1

1 Images

Supplier Data

Western blot - Anti-Wilms Tumor Protein antibody (AB180840)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Wilms Tumor Protein antibody (ab180840) at 1/500 dilution

Lane 1:

A549 cell lysate at 25 µg

Lane 2:

MCF7 cell lysate at 25 µg

Lane 3:

Mouse heart tissue lysate at 25 µg

Lane 4:

Mouse testis tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human WT1.

P19544

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Wilms Tumor Protein commonly known as WT1 is a critical transcription factor implicated in several cellular processes. It is also identified by other names such as Wilms Tumor Suppressor and WT33. WT1 protein has a mass of approximately 52-54 kDa. It is broadly expressed in various tissues with high levels in the developing kidney gonads and certain mesothelial tissues. Its expression plays an important role in organ development and cellular differentiation.

Biological function summary

The WT1 protein regulates gene expression by binding to specific DNA sequences influencing cell growth and differentiation. WT1 acts as a part of larger protein complexes interacting with other transcription factors and co-regulators. It plays essential roles in the development of the urogenital system and in the maintenance of mesothelial cells. The WT1 protein is essential for normal kidney and gonadal development highlighting its significance in embryogenesis.

Pathways

WT1 integration involves multiple cellular signaling cascades. It is notably engaged in the Wnt and PI3K/AKT signaling pathways which are critical for cell proliferation and survival. In these pathways WT1 interaction with proteins such as β-catenin and PI3K subunits modulates cell fate decisions. This involvement in signaling networks exemplifies its importance in cellular homeostasis and response to environmental cues.

WT1 mutations or dysregulation associate strongly with specific pathologies particularly Wilms tumor and acute myeloid leukemia (AML). In Wilms tumor WT1 acts as a tumor suppressor gene and its loss of function leads to tumorigenesis in the kidney. In AML aberrant WT1 expression affects normal hematopoiesis and is often linked to poor prognosis. Interaction with proteins like BSA in immunohistochemistry diagnostics highlights WT1’s significance as a biomarker for these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor that plays an important role in cellular development and cell survival (PubMed : 7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed : 17716689, PubMed : 25258363, PubMed : 7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific : isoforms lacking the KTS motif may act as transcription factors (PubMed : 15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed : 16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed : 19123921).

See full target information WT1

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:101932 PubMed39879993

2025

A functional cardiac patch promotes cardiac repair by modulating the CCR2 cardiac-resident macrophage niche and their cell crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Chengbin Ding,Guofeng Tang,Yan Sun,Xiaodong Fu,Ye Tian,Jiamian Zhan,Songtao Zhang,Xianglong Xing,Jianing Liu,Xiaozhong Qiu,Leyu Wang

Frontiers in oncology 11:744256 PubMed34671560

2021

Patient-Derived Xenografts Are a Reliable Preclinical Model for the Personalized Treatment of Epithelial Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayu Chen,Ying Jin,Siyi Li,Cui Qiao,Xinxin Peng,Yan Li,Yu Gu,Wei Wang,Yan You,Jie Yin,Ying Shan,Yong-Xue Wang,Meng Qin,Hongyue Li,Yan Cai,Yu Dong,Siying Peng,Lingya Pan

Stem cells international 2021:8859071 PubMed33897781

2021

Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Dinghui Wang,Tianhua Xiong,Yajie Liu,Xiaodong Jing,Jianlin Du,Qiang She

American journal of physiology. Renal physiology 320:F548-F558 PubMed33586497

2021

Lipotoxicity dysregulates the immunoproteasome in podocytes and kidneys in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Soon Lee,Ji Yeon Suh,Byeong-Choel Kang,Eugene Lee

Frontiers in cell and developmental biology 9:616711 PubMed33659248

2021

The miR-203a Regulatory Network Affects the Proliferation of Chronic Myeloid Leukemia K562 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua He,Zeping Han,Ziyi An,Yumin Li,Xingyi Xie,Jiabin Zhou,Sihua He,Yubing Lv,Mengling He,Hong Qu,Gexiu Liu,Yuguang Li

BioMed research international 2020:7069052 PubMed33204708

2020

Curcumin Improves the Renal Autophagy in Rat Experimental Membranous Nephropathy via Regulating the PI3K/AKT/mTOR and Nrf2/HO-1 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Di Tu,Juan Jin,Xiao Hu,Yan Ren,Li Zhao,Qiang He

Experimental and therapeutic medicine 19:123-130 PubMed31853281

2019

Resveratrol alleviates obesity-associated podocyte injury in ovariectomized obese rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Li,Xiaoyan Xiao,Yulian Miao,Ling Guo,Junhui Zhen,Xianhua Li,Bei Jiang,Zhao Hu

Endocrinology 161: PubMed31730175

2019

Primary Sertoli Cell Cultures From Adult Mice Have Different Properties Compared With Those Derived From 20-Day-Old Animals.

Applications

Unspecified application

Species

Unspecified reactive species

Arpornrad Saewu,Kessiri Kongmanas,Riya Raghupathy,Jacob Netherton,Suraj Kadunganattil,James-Jules Linton,Watchadaporn Chaisuriyong,Kym F Faull,Mark A Baker,Nongnuj Tanphaichitr

Biochemical pharmacology 166:139-152 PubMed31085161

2019

Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1.

Applications

Unspecified application

Species

Unspecified reactive species

Tu-Shuai Li,Li Chen,Shan-Chun Wang,Yan-Zi Yang,Hong-Jiang Xu,Hong-Mei Gu,Xiao-Juan Zhao,Ping Dong,Ying Pan,Zhan-Qiang Shang,Xi-Quan Zhang,Ling-Dong Kong

Free radical biology & medicine 134:484-497 PubMed30716432

2019

Poricoic acid A enhances melatonin inhibition of AKI-to-CKD transition by regulating Gas6/AxlNFκB/Nrf2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Qian Chen,Ya-Long Feng,Lin Chen,Jing-Ru Liu,Ming Wang,Nosratola D Vaziri,Ying-Yong Zhao

View all publications

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Anti-Wilms Tumor蛋白抗体

Anti-Wilms Tumor Protein antibody

Western blot - Anti-Wilms Tumor Protein antibody (AB180840)