Anti-WHSC1/NSD2抗体(ab225625)
Key features and details
- Rabbit polyclonal to WHSC1/NSD2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WHSC1/NSD2抗体
参阅全部 WHSC1/NSD2 一抗 -
描述
兔多克隆抗体to WHSC1/NSD2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla
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免疫原
Synthetic peptide within Human WHSC1/NSD2 aa 525-575. The exact sequence is proprietary. (NP_579877.1).
Database link: O96028 -
阳性对照
- WB: HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab225625 was affinity purified using an epitope specific to WHSC1/NSD2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225625于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 152 kDa.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 152 kDa. |
靶标
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功能
Probable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription. -
组织特异性
Widely expressed. -
疾病相关
Note=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems. -
序列相似性
Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 HMG box DNA-binding domain.
Contains 4 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 2 PWWP domains.
Contains 1 SET domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm and Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 461070 Chimpanzee
- Entrez Gene: 101140725 Gorilla
- Entrez Gene: 7468 Human
- Omim: 602952 Human
- SwissProt: O96028 Human
- Unigene: 113876 Human
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别名
- FLJ23286 antibody
- IL5 promoter REII region binding protein antibody
- KIAA1090 antibody
see all
图片
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Western blot - Anti-WHSC1/NSD2 antibody (ab225625)All lanes : Anti-WHSC1/NSD2 antibody (ab225625) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Exposure time: 30 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab225625 被引用在 1 文献中.
- Want MY et al. WHSC1/NSD2 regulates immune infiltration in prostate cancer. J Immunother Cancer 9:N/A (2021). PubMed: 33589522