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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other

Anti-Werner's syndrome helicase WRN抗体(ab200)

  • Datasheet
  • SDS
Reviews (4)Q&A (11)References (32)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (ab200)

    Key features and details

    • Rabbit polyclonal to Werner's syndrome helicase WRN
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Werner's syndrome helicase WRN抗体
      参阅全部 Werner's syndrome helicase WRN 一抗
    • 描述

      兔多克隆抗体to Werner's syndrome helicase WRN
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide corresponding to Human Werner's syndrome helicase WRN aa 1-550.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab200于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration.

    靶标

    • 功能

      Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • 疾病相关

      Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • 序列相似性

      Belongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • 翻译后修饰

      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Nucleus > nucleolus. Nucleus.
    • Target information above from: UniProt accession Q14191 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7486 Human
      • Omim: 604611 Human
      • SwissProt: Q14191 Human
      • Unigene: 632050 Human
      • 别名

        • DKFZp686C2056 antibody
        • DNA helicase antibody
        • DNA helicase, RecQ like type 3 antibody
        • Exonuclease WRN antibody
        • HGNC 12791 antibody
        • OTTHUMP00000225301 antibody
        • RecQ protein-like 2 antibody
        • RecQ-like type 3 antibody
        • RecQ3 antibody
        • RECQL2 antibody
        • RECQL3 antibody
        • Werner syndrome ATP-dependent helicase antibody
        • Werner syndrome helicase antibody
        • Werner syndrome protein antibody
        • Werner syndrome, RecQ helicase like antibody
        • WRN antibody
        • WRN_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (ab200)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (ab200)
        ab200 (4µg/ml) staining Werner's syndrome helicase WRN in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and some cytoplasmic staining.
        Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

      实验方案

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (32)

      发表研究结果有使用 ab200?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab200 被引用在 32 文献中.

      • Liu Z  et al. Large-scale chromatin reorganization reactivates placenta-specific genes that drive cellular aging. Dev Cell 57:1347-1368.e12 (2022). WB ; Human . PubMed: 35613614
      • Ogata H  et al. Calcification in Werner syndrome associated with lymphatic vessels aging. Aging (Albany NY) 13:25717-25728 (2021). IHC-P ; Human . PubMed: 34958633
      • Ryu G  et al. Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp. Allergy Asthma Immunol Res 12:507-522 (2020). PubMed: 32141263
      • Veith S  et al. PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner. Sci Rep 9:10075 (2019). PubMed: 31296950
      • Ghosh D  et al. Acetylation of Werner protein at K1127 and K1117 is important for nuclear trafficking and DNA repair. DNA Repair (Amst) 79:22-31 (2019). PubMed: 31085421
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 15 Abreviews or Q&A

      Western blot abreview for Anti-Werners syndrome helicase WRN antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      HeLa
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 13 2017

      Western blot abreview for Anti-Werners syndrome helicase WRN antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing (4-16% gradient Tris-Gly gel)
      Sample
      Mouse Cell lysate - whole cell (MEFs)
      Specification
      MEFs
      Blocking step
      LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 17 2015

      Western blot abreview for Anti-Werners syndrome helicase WRN antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing (4-16% gradient Tris-Gly gel)
      Sample
      Human Cell lysate - whole cell (Human primary fibroblasts)
      Specification
      Human primary fibroblasts
      Blocking step
      LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 16 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Werner's syndrome helicase WRN antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Methanol
      Permeabilization
      Yes - 0.5% Triton X100 in PBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Kirk Mcmanus

      Verified customer

      提交于 Oct 30 2007

      Question

      We tried both antibodies again. WRN-ab 17987 did not recognize any protein band with all the controls.The BLM-ab2179 simply recognized a whole lot of proteins--so we just cannot tell which of these would be specific for BLM. I think is best to provide a credit as we will soon need to order anti-histone antibodies.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 12 2012

      Answer

      Thank you for your reply.
      Your credit note ID is ********.
      I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.
      Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
      The credit note ID is for your reference only and does not automatically guarantee the credit.
      I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

      Read More

      Abcam Scientific Support

      回复于 Jun 12 2012

      Question

      . Please send WRN ab17987 and BLM ab2179.
      Kind regards

      Read More

      Abcam community

      Verified customer

      Asked on Jun 04 2012

      Answer

      Thank you for your reply.
      There will be a delay in you receiving these antibodies as ab2179 is currently out of stock, but it is due to come in on June 11th and we will hopefully send it to you the same day. I am sorry about the delay.
      Your new order number for tracking purposes is Order ************.
      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      回复于 Jun 04 2012

      Question

      No signal in western blot, only high background.

      Read More

      Abcam community

      Verified customer

      Asked on May 31 2012

      Answer

      Thank you for calling Abcam earlier today.
      I am sorry about the difficulty you have been having with a few of our antibodies and below are links to a few that would make a suitable alternative:
      - https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-ab17987.html
      - https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-EPR6392-ab124673.html
      - https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab2179.html
      - https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab86664.html
      Please let me know which antibodies you would like as replacements and I will have them sent to you as soon as possible.

      Read More

      Abcam Scientific Support

      回复于 Jun 01 2012

      Question

      An article about ab200, publicated in PNAS 2006, showed the results of immunohistochemistry (IHC) of WRN protein. But the method was not explained in the article, and IHC is not listed in your product catalogue. How can I get the method of IHC of Werner protein using ab200?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 07 2006

      Answer

      Thank you for your enquiry. You are correct the publication that you refer to; Agrelo R et al. Epigenetic inactivation of the premature aging Werner syndrome gene in human cancer. Proc Natl Acad Sci U S A 103:8822-7 (2006). PubMed: 16723399 applied this antibody in cultured cell lines using immunofluorescence in addition to immunohistochemistry on Werner's syndrome mice. In the publication within the methods section they refer to references 22 and 30 for their methodology in these two applications; Ropero, S., Setien, F., Espada, J., Fraga, M. F., Herranz, M., Asp, J., Benassi, M. S., Franchi, A., Patino, A., Ward, L. S., et al. (2004) Hum. Mol. Genet. 13, 2753–2765. Chen, L., Lee, L., Kudlow, B. A., Dos Santos, H. G., Sletvold, O., Shafeghati, Y., Botha, E. G., Garg, A., Hanson, N. B., Martin, G. M., et al. (2003) Lancet 362, 440–445. Unfortunately these two pubclications do in fact refer to; BK Kennedy, DA Barbie, M Classon, N Dyson and E Harlow, Nuclear organization of DNA replication in primary mammalian cells, Genes Dev 14 (2000), pp. 2855–2868 in their methods section. However, I have examined this publication and this does not detail the approach used; whether frozen sections or paraffin embedded sections. Therefore unfortunately I cannot update this antibody datasheet to reflect the antibody's application in this publication. For details of the methods employed I would encourage you to contact them directly using the email address detailed on the publications. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Oct 09 2006

      Question

      Can you tell me the immunogen used to make this antibody? I would like to know if it can react with a truncated form of the protein I am studying.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 02 2006

      Answer

      Thank you for your enquiry. The immunogen used to make this antibody was the N-terminus, amino acids 1-510, of the protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Jun 06 2006

      Question

      BATCH NUMBER 34820 DESCRIPTION OF THE PROBLEM also batch 79641. High background. SAMPLE HeLa whole cell extract PRIMARY ANTIBODY 1:500 ON at 4C in TBS SECONDARY ANTIBODY 1 hr at RT at 1:10000 in TBST DETECTION METHOD Supersignal POSITIVE AND NEGATIVE CONTROLS USED HeLa WCE No primary gave no background. TRANSFER AND BLOCKING CONDITIONS 7.5% polyacrylamide (37.5:1) Bis 30 min, 50 volts, transfer to PVDF membrane 5% NFDM in TBST HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? numer HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes WHAT STEPS HAVE YOU ALTERED? Primary at 1:500, 1:1000, 1:2000 Block in NFDM at 5% and 10% in TBST Block in 5% BSA in TBST ADDITIONAL NOTES Have used successflly in the past but the latest lots, 34820 and 79641, have had excessive background. Some suggestions reduced background but also reduced signal to unsatisfactory levels.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 17 2005

      Answer

      Thank you for your enquiry and for taking your time to fill in the on-line questionnaire. We are very sorry to her that your customer is having problem with this antibody. Would you be so kind to provide further information about: 1. How much protein was loaded onto the gel? 2. What is the secondary antibody? In what species was it raised? We certainly can offer you a new vial free of charge. Please do let us know if your customer would like to test it. We look forward to hearing from you soon.

      Read More

      Abcam Scientific Support

      回复于 Mar 18 2005

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