Anti-Werner's syndrome helicase WRN抗体(ab200)

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Thank you for your reply.
There will be a delay in you receiving these antibodies as ab2179 is currently out of stock, but it is due to come in on June 11th and we will hopefully send it to you the same day. I am sorry about the delay.
Your new order number for tracking purposes is Order ************.
If there is anything else I can help you with, please let me know.

Thank you for calling Abcam earlier today.
I am sorry about the difficulty you have been having with a few of our antibodies and below are links to a few that would make a suitable alternative:
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-ab17987.html
- https://www.abcam.com/Werner-s-syndrome-helicase-WRN-antibody-EPR6392-ab124673.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab2179.html
- https://www.abcam.com/Blooms-Syndrome-Protein-Blm-antibody-ab86664.html
Please let me know which antibodies you would like as replacements and I will have them sent to you as soon as possible.

Thank you for your enquiry. You are correct the publication that you refer to; Agrelo R et al. Epigenetic inactivation of the premature aging Werner syndrome gene in human cancer. Proc Natl Acad Sci U S A 103:8822-7 (2006). PubMed: 16723399 applied this antibody in cultured cell lines using immunofluorescence in addition to immunohistochemistry on Werner's syndrome mice. In the publication within the methods section they refer to references 22 and 30 for their methodology in these two applications; Ropero, S., Setien, F., Espada, J., Fraga, M. F., Herranz, M., Asp, J., Benassi, M. S., Franchi, A., Patino, A., Ward, L. S., et al. (2004) Hum. Mol. Genet. 13, 2753–2765. Chen, L., Lee, L., Kudlow, B. A., Dos Santos, H. G., Sletvold, O., Shafeghati, Y., Botha, E. G., Garg, A., Hanson, N. B., Martin, G. M., et al. (2003) Lancet 362, 440–445. Unfortunately these two pubclications do in fact refer to; BK Kennedy, DA Barbie, M Classon, N Dyson and E Harlow, Nuclear organization of DNA replication in primary mammalian cells, Genes Dev 14 (2000), pp. 2855–2868 in their methods section. However, I have examined this publication and this does not detail the approach used; whether frozen sections or paraffin embedded sections. Therefore unfortunately I cannot update this antibody datasheet to reflect the antibody's application in this publication. For details of the methods employed I would encourage you to contact them directly using the email address detailed on the publications. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. The immunogen used to make this antibody was the N-terminus, amino acids 1-510, of the protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry and for taking your time to fill in the on-line questionnaire. We are very sorry to her that your customer is having problem with this antibody. Would you be so kind to provide further information about: 1. How much protein was loaded onto the gel? 2. What is the secondary antibody? In what species was it raised? We certainly can offer you a new vial free of charge. Please do let us know if your customer would like to test it. We look forward to hearing from you soon.