Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-WDTC1抗体[EPR12504] (ab174294)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] (ab174294)
  • Western blot - Anti-WDTC1 antibody [EPR12504] (ab174294)
  • Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (ab174294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)
  • Anti-WDTC1 antibody [EPR12504] (ab174294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12504] to WDTC1
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-WDTC1抗体[EPR12504]
    参阅全部 WDTC1 一抗

  • 描述

    兔单克隆抗体[EPR12504] to WDTC1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, IP, ICC/IFmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human WDTC1. The exact sequence is proprietary.
    Database link: Q8N5D0

  • 阳性对照

    • HepG2 and A431 cell lysates. Human liver and lung carcinoma tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12504

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab174294于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
说明
WB
1/10000 - 1/50000. Predicted molecular weight: 76 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
ICC/IF
1/50 - 1/100.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 相关性

    WDTC1 contains 2 TPR repeats and 7 WD repeats. WDTC1, the ortholog of Drosophila Adipose Gene, associates with human obesity, modulated by MUFA intake. There are six named isoforms.

  • 细胞定位

    Cytosol. Nucleus.

  • 数据库链接

    • 别名

      • Adipose homolog antibody
      • ADP antibody
      • DCAF9 antibody
      • DDB1 and CUL4 associated factor 9 antibody
      • KIAA1037 antibody
      • WD and tetratricopeptide repeats 1 antibody
      • WD and tetratricopeptide repeats protein 1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] (ab174294)
      Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] (ab174294)

      Ab174294 staining WDTC1 in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/50 dilution (1.6 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2 μg/ml. An AlexaFluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker, ab195889 was used as a counterstain antibody (1/200, 2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line.

    • Western blot - Anti-WDTC1 antibody [EPR12504] (ab174294)
      Western blot - Anti-WDTC1 antibody [EPR12504] (ab174294)
      All lanes : Anti-WDTC1 antibody [EPR12504] (ab174294) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 76 kDa

    • Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (ab174294)
      Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (ab174294)

      Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate  immunoprecipitated using ab174294 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling WDTC1 with ab174294 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (ab174294)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling WDTC1 with ab174294 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-WDTC1 antibody [EPR12504] (ab174294)
      Anti-WDTC1 antibody [EPR12504] (ab174294)

    文献 (1)

    发表研究结果有使用 ab174294？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab174294 被引用在 1 文献中.

    • Groh BS  et al. The antiobesity factor WDTC1 suppresses adipogenesis via the CRL4WDTC1 E3 ligase. EMBO Rep 17:638-47 (2016). PubMed: 27113764

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab174294.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com