AB174294

重组Anti-WDTC1抗体[EPR12504]

Anti-WDTC1 antibody [EPR12504]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal WDTC1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

KIAA1037, WDTC1, WD and tetratricopeptide repeats protein 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (AB174294)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (AB174294)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling WDTC1 with ab174294 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (AB174294)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling WDTC1 with ab174294 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] (AB174294)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] (AB174294)

ab174294 staining WDTC1 in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/50 dilution (1.6 μg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2 μg/ml. An AlexaFluor^® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used as a counterstain antibody (1/200 2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line.

Supplier Data

Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (AB174294)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab174294 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (ab174294)

Predicted band size: 76 kDa

false

Supplier Data

Western blot - Anti-WDTC1 antibody [EPR12504] (AB174294)

All lanes:

Western blot - Anti-WDTC1 antibody [EPR12504] (ab174294) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 76 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-WDTC1 antibody [EPR12504] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12504

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

WDTC1 also known as Adipose is a protein that plays a role in various cellular processes. It is encoded by the WDTC1 gene and has a molecular weight of approximately 67 kDa. WDTC1 displays expression across many tissues with notable presence in adipose tissue and skeletal muscle. The protein contains WD40 repeats and a TBC (Tre-2/Bub2/Cdc16) domain which assist in protein-protein interactions and cellular trafficking.

Biological function summary

WDTC1 influences energy regulation and fat metabolism. It forms part of a protein complex that includes CUL4-DDB1 contributing to the ubiquitin-proteasome system. This activity regulates degradation of selective proteins ultimately controlling fat storage and mobilization. WDTC1 affects adipogenesis by modulating transcription factors and is involved in lipid metabolism through interactions with other cellular proteins.

Pathways

WDTC1 is actively involved in the regulation of metabolic pathways. It engages with the insulin signaling pathway and the PPARγ (peroxisome proliferator-activated receptor gamma) pathway both essential for energy homeostasis and fat cell differentiation. WDTC1 interacts with insulin receptor substrate proteins and partners with other ligase components to mediate the effects of these pathways on metabolism.

WDTC1 connects to obesity and type 2 diabetes. Variations in WDTC1 expression and function can disrupt normal fat metabolism contributing to these metabolic disorders. It associates with the hormone leptin a critical player in maintaining body weight linking WDTC1 to adipose tissue dysfunction. Understanding the role of WDTC1 may offer insight into therapeutic targets for metabolic disease management.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

See full target information WDTC1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:7280312 PubMed35498136

2022

Screening of lncRNA-miRNA-mRNA Coexpression Regulatory Networks Involved in Acute Traumatic Coagulation Dysfunction Based on CTD, GeneCards, and PharmGKB Databases.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Luo,Yong Fu,Taifa Tan,Jun Hu,Fang Li,Zhanchen Liao,Jian Peng

EMBO reports 17:638-47 PubMed27113764

2016

The antiobesity factor WDTC1 suppresses adipogenesis via the CRL4WDTC1 E3 ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Beezly S Groh,Feng Yan,Matthew D Smith,Yanbao Yu,Xian Chen,Yue Xiong

View all publications

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重组Anti-WDTC1抗体[EPR12504]