Anti-WDR91抗体(ab80614)
Key features and details
- Rabbit polyclonal to WDR91
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-WDR91抗体
参阅全部 WDR91 一抗 -
描述
兔多克隆抗体to WDR91 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey, Gorilla
-
免疫原
Synthetic peptide corresponding to a region between residues 350 and 400 of human WDR91 (NP_054868.2).
-
阳性对照
- HeLa whole cell lysate. 293T whole cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80614于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
序列相似性
Contains 7 WD repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
-
数据库链接
- Entrez Gene: 29062 Human
- SwissProt: A4D1P6 Human
- Unigene: 459858 Human
-
别名
- HSPC049 antibody
- WD repeat containing protein 91 antibody
- WD repeat domain 91 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR91 antibody (ab80614)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection: DAB.
-
Western blot - Anti-WDR91 antibody (ab80614)All lanes : Anti-WDR91 antibody (ab80614) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-WDR91 antibody (ab80614)Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3µg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1µg/ml and detected with chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab80614 尚未被引用在任何文献中。