AB80614

Anti-WDR91抗体

Name: WDR91抗体 (ab80614)| Abcam中文官网
Brand: Abcam Limited
SKU: AB80614
Price: 4606 CNY
Availability: InStock

Anti-WDR91 antibody

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(1 Publication)

Rabbit Polyclonal WDR91 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WDR91.

查看别名

HSPC049, WDR91, WD repeat-containing protein 91

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR91 antibody (AB80614)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR91 antibody (AB80614)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling WDR91 with ab80614 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-WDR91 antibody (AB80614)

Immunoprecipitation of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab80614 at 3μg/mg lysate (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80614 at 1μg/ml and detected with chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-WDR91 antibody (ab80614)

Predicted band size: 83 kDa

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Unknown

Western blot - Anti-WDR91 antibody (AB80614)

All lanes:

Western blot - Anti-WDR91 antibody (ab80614) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 83 kDa

Observed band size: 83 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP, IHC-P

applications

免疫原

Synthetic Peptide within Human WDR91. The exact immunogen used to generate this antibody is proprietary information.

A4D1P6

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The WDR91 protein also known as WD repeat domain 91 functions mechanistically by interacting with phosphatidylinositol 3-phosphate a lipid important for cellular processes. WDR91 has approximately a molecular weight of 90 kDa. It is expressed in various tissues with high levels found in the brain where it plays a significant role in cellular mechanisms. It contains WD40 repeats known for facilitating protein-protein interactions which are vital for its function in cellular processes.

Biological function summary

WDR91 facilitates endosomal signaling and trafficking processes. It assists in the proper sorting and recycling of receptors that regulate cellular responses. WDR91 forms part of a protein complex that includes key players like PIKFYVE which influences lipid synthesis on endosomal membranes. This involvement highlights its role in maintaining balanced cellular responses to environmental signals impacting overall cellular health.

Pathways

The activities of WDR91 integrate into the endosomal sorting and phosphoinositide signaling pathways. In the endosomal sorting pathway it collaborates with proteins like VPS34 and VPS15 to regulate endosome maturation. Through its function in phosphoinositide signaling it impacts aspects of cellular metabolism and organization affecting pathways linked to cellular growth and survival.

WDR91 is related to a few neurological conditions. Mutations or dysregulation of WDR91 contributes to neurodegenerative disorders such as Alzheimer’s Disease. It associates with proteins like PIKFYVE in disease contexts where disruptions can lead to the accumulation of undefined cellular debris. A better understanding of WDR91 in these disease pathways may offer new insights into potential therapeutic approaches for such debilitating conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as a negative regulator of the PI3 kinase/PI3K activity associated with endosomal membranes via BECN1, a core subunit of the PI3K complex. By modifying the phosphatidylinositol 3-phosphate/PtdInsP3 content of endosomal membranes may regulate endosome fusion, recycling, sorting and early to late endosome transport (PubMed : 26783301). It is for instance, required for the delivery of cargos like BST2/tetherin from early to late endosome and thereby participates indirectly to their degradation by the lysosome (PubMed : 27126989). May play a role in meiosis (By similarity).

See full target information WDR91

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110418 PubMed40570961

2025

Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpu Xue,Gabriel P Faber,Lea S Pommerening,Megha Mallick,Aditi Gupta,Markus C Wahl,Yaron Shav-Tal,Sutapa Chakrabarti

View all publications

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Anti-WDR91抗体

Anti-WDR91 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR91 antibody (AB80614)

Immunoprecipitation - Anti-WDR91 antibody (AB80614)

Western blot - Anti-WDR91 antibody (AB80614)