Anti-WDR5抗体[2C2] (ab56919)
Key features and details
- Mouse monoclonal [2C2] to WDR5
- Suitable for: WB, ICC/IF, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-WDR5抗体[2C2]
参阅全部 WDR5 一抗 -
描述
小鼠单克隆抗体[2C2] to WDR5 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, ChIP, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5
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常规说明
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4 -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
2C2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56919于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 20864036
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 20864036 |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
序列相似性
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 11091 Human
- Omim: 609012 Human
- SwissProt: P61964 Human
- Unigene: 397638 Human
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别名
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
图片
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WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate
Predicted band size: 36 kDaThis image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (35)
ab56919 被引用在 35 文献中.
- Zhou T et al. Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Nat Commun 13:1855 (2022). PubMed: 35388006
- Zhang C et al. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury. Cell Death Dis 13:770 (2022). PubMed: 36068197
- Ka M et al. WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling. Mol Neurobiol 59:5104-5120 (2022). PubMed: 35672601
- Fischer V et al. The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms. Cell Rep 36:109598 (2021). PubMed: 34433046
- Yang B et al. Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly. J Virol 95:N/A (2021). PubMed: 33504601