Anti-WDR42A抗体(ab176809)
Key features and details
- Rabbit polyclonal to WDR42A
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-WDR42A抗体 -
描述
兔多克隆抗体to WDR42A -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
-
免疫原
Synthetic peptide within Human WDR42A aa 50-100. The exact sequence is proprietary. (NP_056541.2).
Sequence:LTGDDGGPNRTSTESRGTDTESSGEDKDSDSMEDTGHYSINDENRVHDRS E
Database link: Q5TAQ9 -
阳性对照
- HeLa and 293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176809 was affinity purified using an epitope specific to WDR42A immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176809于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
|
| 说明 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 67 kDa. |
靶标
-
相关性
This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. The protein contains 7 WD repeats. There are two isoforms produced by alternative splicing. -
数据库链接
- Entrez Gene: 457434 Chimpanzee
- Entrez Gene: 510316 Cow
- Entrez Gene: 101144021 Gorilla
- Entrez Gene: 50717 Human
- Entrez Gene: 98193 Mouse
- Entrez Gene: 100174058 Orangutan
- Entrez Gene: 100153655 Pig
- Entrez Gene: 364050 Rat
see all -
别名
- DCAF8 antibody
- DDB1 and CUL4 associated factor 8 antibody
- DKFZp781G1096 antibody
see all
图片
-
Western blot - Anti-WDR42A antibody (ab176809)All lanes : Anti-WDR42A antibody (ab176809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 3 seconds -
Immunoprecipitation - Anti-WDR42A antibody (ab176809)Detection of WDR42A in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176809 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
-
Datasheet download
文献 (0)
ab176809 尚未被引用在任何文献中。
