Anti-WDR33抗体(ab72115)
Key features and details
- Rabbit polyclonal to WDR33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WDR33抗体
参阅全部 WDR33 一抗 -
描述
兔多克隆抗体to WDR33 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72115于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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组织特异性
Most highly expressed in testis. -
序列相似性
Contains 1 collagen-like domain.
Contains 7 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55339 Human
- SwissProt: Q6NUQ0 Human
- SwissProt: Q9C0J8 Human
- Unigene: 554831 Human
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别名
- WD repeat containing protein 33 antibody
- WD repeat domain 33 antibody
- WD repeat-containing protein 33 antibody
see all
图片
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Western blot - Anti-WDR33 antibody (ab72115)All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 146 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Immunoprecipitation - Anti-WDR33 antibody (ab72115)Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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