Anti-WDR13抗体(ab272543)
Key features and details
- Rabbit polyclonal to WDR13
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-WDR13抗体 -
描述
兔多克隆抗体to WDR13 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Orangutan
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免疫原
Recombinant fragment corresponding to Human WDR13 aa 100-250.
Database link: Q9H1Z4 -
阳性对照
- WB: Human cell line RT-4 IHC-P: Human fallopian tissue ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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组织特异性
Widely expressed. -
序列相似性
Contains 5 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 64743 Human
- Entrez Gene: 73447 Mouse
- Omim: 300512 Human
- SwissProt: Q9H1Z4 Human
- SwissProt: Q91V09 Mouse
- Unigene: 521973 Human
- Unigene: 284162 Mouse
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别名
- DKFZp779C2057 antibody
- FLJ20563 antibody
- located at OATL1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR13 antibody (ab272543)
Paraffin-embedded formalin-fixed Human fallopian tissue stained for WDR13 using ab272543 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-WDR13 antibody (ab272543)Lane 2 : Anti-WDR13 antibody (ab272543) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Human cell line RT-4 -
Immunocytochemistry/ Immunofluorescence - Anti-WDR13 antibody (ab272543)PFA fixed, Triton X-100 permeablized human cell line A-431 labeling WDR13 (green) using ab272543 at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR13 antibody (ab272543)Paraffin-embedded formalin-fixed Human tonsil tissue stained for WDR13 using ab272543 1/500 dilution in immunohistochemical analysis. Shows low expression as expected.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272543 尚未被引用在任何文献中。