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重组RabMAb

重组Anti-WDR1抗体[EPR8793] (ab173574)

Submit a review Submit a question References (4)
Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
  • Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)
  • Anti-WDR1 antibody [EPR8793] (ab173574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8793] to WDR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-WDR1抗体[EPR8793]
    参阅全部 WDR1 一抗

  • 描述

    兔单克隆抗体[EPR8793] to WDR1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow

  • 免疫原

    Synthetic peptide within Human WDR1 aa 300-400. The exact sequence is proprietary.
    Database link: O75083

  • 阳性对照

    • WB: Human heart tissue lysate. Jurkat, Raji, HT-1080 and U87-MG cell lysates. IHC: Human skeletal muscle tissue and endometrial carcinoma tissue. ICC/IF: Raji cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR8793

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab173574于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

图片

  • Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/200 dilution + Human heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Buffer concentration: 5% NFDM /TBST. 

    For human samples, please use lower antibody dilution or upload more of the protein.

  • Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    All lanes : Anti-WDR1 antibody [EPR8793] (ab173574) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Buffer concentration: 5% NFDM /TBST 

    We are unsure as to the identity of the extra band below 75kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

  • Anti-WDR1 antibody [EPR8793] (ab173574)
    Anti-WDR1 antibody [EPR8793] (ab173574)

文献 (4)

发表研究结果有使用 ab173574？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab173574 被引用在 4 文献中.

  • Yao W  et al. TNK2-AS1 upregulated by YY1 boosts the course of osteosarcoma through targeting miR-4319/WDR1. Cancer Sci 112:893-905 (2021). PubMed: 33164271
  • Iqbal J  et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). PubMed: 30054946
  • López-Coral A  et al. A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion. Sci Rep 8:13049 (2018). PubMed: 30158666
  • Hong F  et al. Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90. PLoS One 12:e0169260 (2017). PubMed: 28056051

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