AB224221

Anti-WDHD1抗体

Name: WDHD1抗体 (ab224221)| Abcam中文官网
Brand: Abcam Limited
SKU: AB224221
Price: 5757 CNY
Availability: InStock

Anti-WDHD1 antibody

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal WDHD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDHD1 aa 200-350.

查看别名

AND1, WDHD1, WD repeat and HMG-box DNA-binding protein 1, Acidic nucleoplasmic DNA-binding protein 1, And-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for WDHD1 (green) using ab224221 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

Paraffin-embedded human tonsil tissue stained for WDHD1 using ab224221 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 126 kDa

true

Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 126 kDa

true

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human WDHD1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

O75717

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork.

See full target information WDHD1

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2383886 PubMed39072709

2024

Design, synthesis, and evaluation of novel stilbene derivatives that degrade acidic nucleoplasmic DNA-binding protein 1 (And1) and synergize with PARP1 inhibitor in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leyuan Chen,Zhonghao Ren,Yunze Zhang,Wenbin Hou,Yiliang Li

FEBS open bio 13:102-117 PubMed36345604

2022

WDHD1 is over-expressed in nasopharyngeal carcinoma and may control the expression of ITGAV.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yun Wu,Yi-Tong Niu,Su-Ning Huang,Yu-Min Tan,Zhen-Dong Yang,Ye-Ying Fang,Li Jiang,Ting-Ting Zhang,Xiao-Fen Zeng,Yun-Xi Peng,Miao Mo,Cai-Xing Lin,Zhu-Xin Wei

Nature communications 13:6090 PubMed36241664

2022

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri Lukas

Science (New York, N.Y.) 369: PubMed32973005

2020

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong Park

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDHD1抗体

Anti-WDHD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

Western blot - Anti-WDHD1 antibody (AB224221)

Western blot - Anti-WDHD1 antibody (AB224221)