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AB185546

重组Anti-WAVE 1抗体[EPR15021] - N-terminal

Anti-WAVE 1 antibody [EPR15021] - N-terminal

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(2 Publications)

Rabbit Recombinant Monoclonal WAVE 1 antibody. N-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

KIAA0269, SCAR1, WAVE1, WASF1, Actin-binding protein WASF1, Protein WAVE-1, Verprolin homology domain-containing protein 1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1

2 Images
Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

Intracellular Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)
  • WB

Supplier Data

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

All lanes:

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 80 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-WAVE 1 antibody [EPR15021] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15021

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation (PubMed : 29961568). The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). Also involved in the regulation of mitochondrial dynamics (PubMed : 29961568).
See full target information WASF1

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed39724071

2024

Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Saarela,Pawel Lis,Sara Gomes,Raja S Nirujogi,Wentao Dong,Eshaan Rawat,Sophie Glendinning,Karolina Zeneviciute,Enrico Bagnoli,Rotimi Fasimoye,Cindy Lin,Kwamina Nyame,Fanni A Boros,Friederike Zunke,Frederic Lamoliatte,Sadik Elshani,Matthew Jaconelli,Judith Jm Jans,Margriet A Huisman,Christian Posern,Lena M Westermann,Angela Schulz,Peter M van Hasselt,Dario R Alessi,Monther Abu-Remaileh,Esther M Sammler

Cell proliferation 55:e13169 PubMed34957619

2021

Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Han,Feiyu Wang,Yulu Yue,Xihong Tan,Miao Tian,Yiliang Miao,Shuhong Zhao,Weijie Dong,Mei Yu
View all publications

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