Anti-WAVE 1抗体(ab272916)
Key features and details
- Rabbit polyclonal to WAVE 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-WAVE 1抗体
参阅全部 WAVE 1 一抗 -
描述
兔多克隆抗体to WAVE 1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Mouse WAVE 1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q8R5H6 -
阳性对照
- WB: Mouse and rat brain tissue extracts. ICC/IF: SK-N-SH cells, DIV9 rat E18 primary cortical neuron cells. IHC-P: Mouse and rat brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. -
组织特异性
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood. -
序列相似性
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain. -
结构域
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain. -
细胞定位
Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas. - Information by UniProt
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数据库链接
- Entrez Gene: 8936 Human
- Entrez Gene: 83767 Mouse
- Entrez Gene: 294568 Rat
- Omim: 605035 Human
- SwissProt: Q92558 Human
- SwissProt: Q8R5H6 Mouse
- SwissProt: Q5BJU7 Rat
- Unigene: 75850 Human
see all -
别名
- WASP family, verprolin homology domain-containing protein 1 antibody
- WASP family member 1 antibody
- FLJ31482 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)
4% PFA-fixed SK-N-SH (Human neuroblastoma cell line) cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)Paraffin-embedded mouse brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-WAVE 1 antibody (ab272916)All lanes : Anti-WAVE 1 antibody (ab272916) at 1/1000 dilution
Lane 1 : Mouse brain tissue extracts
Lane 2 : Rat brain tissue extracts
Lysates/proteins at 50 µg per lane. -
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)4% PFA-fixed DIV9 rat E18 primary cortical neuron cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Red: Beta tubulin.
Blue: DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)Paraffin-embedded rat brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272916 尚未被引用在任何文献中。