重组Anti-WAPL/FOE抗体[EPR3407(2)] (ab109537)
![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.cn/ps/products/109/ab109537/Images/ab109537-122482-anti-wapl-antibody-epr34072-western-blot.jpg "Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3407(2)] to WAPL/FOE
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-WAPL/FOE抗体[EPR3407(2)]
参阅全部 WAPL/FOE 一抗 -
描述
兔单克隆抗体[EPR3407(2)] to WAPL/FOE -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, 293T, and Jurkat cell lysates. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3407(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/50000 - 1/200000. Predicted molecular weight: 133 kDa.
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| ICC/IF |
1/100 - 1/250.
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| 说明 |
|---|
|
WB
1/50000 - 1/200000. Predicted molecular weight: 133 kDa. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt or IHC-P.
靶标
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功能
Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
组织特异性
Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. -
序列相似性
Contains 1 WAPL domain. -
细胞定位
Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase. - Information by UniProt
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数据库链接
- Entrez Gene: 23063 Human
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- Unigene: 203099 Human
- Unigene: 714876 Human
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别名
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
图片
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Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 133 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.cn/ps/products/109/ab109537/Images/ab109537-122481-anti-wapl-antibody-epr34072-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
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![Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.cn/ps/products/109/ab109537/Images/ab109537-122480-anti-wapl-antibody-epr34072-western-blot.jpg)
Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)All lanes : Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution
Lane 1 : Human U2OS cells treated with siRNA control.
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL/FOE
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL/FOE
Lysates/proteins at 90 µg per lane.
Secondary
Lane 1 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Lanes 2-3 : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa (possible cross reactivity), 130 kDa (possible cross reactivity), 145 kDa (possible cross reactivity)
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![Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)](https://www.abcam.cn/ps/products/109/ab109537/Images/ab109537-4-benefits-of-recombinant-antibodies.png)
Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab109537 被引用在 2 文献中.
- Pinzaru AM et al. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Genes Dev 34:1619-1636 (2020). PubMed: 33122293
- Kanke M et al. Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO J 35:2686-2698 (2016). PubMed: 27872142