AB109537

重组Anti-WAPL/FOE抗体[EPR3407(2)]

Name: WAPL/FOE抗体[EPR3407(2)] (ab109537)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109537
Price: 2711 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-WAPL/FOE antibody [EPR3407(2)]

RabMAb
Recombinant
了解详情

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal WAPL/FOE antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

FOE, KIAA0261, WAPAL, WAPL, Wings apart-like protein homolog, Friend of EBNA2 protein, WAPL cohesin release factor

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

AbReview32141****

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

Lane 1:

Human U2OS cells treated with siRNA control. at 90 µg

Lane 2:

Human U2OS cells treated with siRNA #1 against WAPL/FOE at 90 µg

Lane 3:

Human U2OS cells treated with siRNA #2 against WAPL/FOE at 90 µg

Secondary

All lanes:

Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 120 kDa,130 kDa,145 kDa,150 kDa

true

Unknown

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 133 kDa,74 kDa

Observed band size: 70 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-WAPL/FOE antibody [EPR3407(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3407(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin (PubMed : 26299517). Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

See full target information WAPL

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

The EMBO journal 35:2686-2698 PubMed27872142

2016

Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Kanke,Eri Tahara,Pim J Huis In't Veld,Tomoko Nishiyama

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-WAPL/FOE抗体[EPR3407(2)]