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Tags & Cell Markers Epitope Tags Other Tags VSV-G Tag

Anti-VSV-G tag抗体(ab1874)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (16)

Product price, shipping and contact information

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Key features and details

  • Rabbit polyclonal to VSV-G tag
  • Suitable for: ICC, WB, IP, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-VSV-G tag antibody [EPR12997] (ab183497)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-VSV-G tag抗体
    参阅全部 VSV-G tag 一抗
  • 描述

    兔多克隆抗体to VSV-G tag
  • 宿主

    Rabbit
  • 特异性

    This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
  • 经测试应用

    适用于: ICC, WB, IP, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:

    YTDIEMNRLGK

    .
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • VSV-G Tag

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1874于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
1/100.
WB (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
1/200.
说明
ICC
1/100.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
1/200.

靶标

  • 相关性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 别名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

实验方案

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (16)

发表研究结果有使用 ab1874?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1874 被引用在 16 文献中.

  • Voss TD  et al. Ubiquitination and the proteasome rather than caspase-3-mediated C-terminal cleavage are involved in the EAAT2 degradation by staurosporine-induced cellular stress. J Neurochem 157:1284-1299 (2021). PubMed: 33180957
  • Xu J  et al. The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NF?B transcripts to dampen antiviral innate immunity. PLoS Pathog 17:e1009530 (2021). PubMed: 33909701
  • Cui S  et al. Nuclear cGAS Functions Non-canonically to Enhance Antiviral Immunity via Recruiting Methyltransferase Prmt5. Cell Rep 33:108490 (2020). PubMed: 33296647
  • Ni YX  et al. A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating. Cell Discov 4:65 (2018). PubMed: 30603101
  • Dutta P  et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . PubMed: 29496607
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry abreview for Anti-VSV-G tag antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Saccharomyces cerevisiae Cell
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Chesneau Laurent

Verified customer

提交于 Sep 19 2005

Western blot abreview for Anti-VSV-G tag antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Chesneau Laurent

Verified customer

提交于 Sep 02 2005

Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

Read More

Abcam community

Verified customer

Asked on Mar 07 2012

Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

Read More

Abcam Scientific Support

回复于 Mar 07 2012

Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

The antibody has not been tested in this way so we do not know it it will bind.

Read More

Asdf Edo

Abcam Scientific Support

回复于 Oct 13 2003

Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

Read More

Asdf Edo

Abcam Scientific Support

回复于 Oct 13 2003

Question

Phone call requesting information on antibodies directed against the VSV-G tag for use in IHC.

Read More

Abcam community

Verified customer

Asked on Oct 09 2012

Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have looked into the antibodies we have which are directed against the VSV-G tag. As you have seen, the reference where ab1874 was used in the staining of frozen rat lung tissue were not used to detect the epitope directly as part of the tissue. It was used to detect and antibody directed against heparan sulfate which contained the tag. The antibody was therefore used more like a secondary antibody.

Unfortunately, we do not have any antibodies where the VSV-G epitope has been detected for IHC as yet. This does not mean that the antibodies would not work in this application, simply that we have not tested it ourselves and would not be able to guarantee its performance.

I am however able to offer you a testing discount if you would like to try one of our antibodies. This offer means that if you purchase an antibody such as the rabbit polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab1874.html or chicken polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab27026.html as normal, test the antibody with your mouse samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the value of the antibody tested off any future purchase with Abcam. More information on this offer can be found from the following link:

https://www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of the antibody to be tested.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Abcam Scientific Support

回复于 Oct 09 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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