重组Anti-VSIG4抗体[EPR22576-70] (ab252933)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22576-70] to VSIG4
- Suitable for: IHC-Fr, WB, IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-VSIG4抗体[EPR22576-70]
参阅全部 VSIG4 一抗 -
描述
兔单克隆抗体[EPR22576-70] to VSIG4 -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human liver and placenta tissue lysate; Jurkat and THP-1 cell lysate; Mouse liver tissue lysate.His-tagged human VSIG4 recombinant protein (aa 19-232). IHC-P: Human liver, placenta and lung tissue; Mouse lung tissue. IHC-Fr: Mouse liver tissue. IP: Human placenta lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22576-70 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab252933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/100.
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WB |
1/1000. Detects a band of approximately 37, 50 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/500.
Perform heat mediated antigen retrieval using ab208572 retrieval buffer. |
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IP |
1/30.
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说明 |
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IHC-Fr
1/100. |
WB
1/1000. Detects a band of approximately 37, 50 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval using ab208572 retrieval buffer. |
IP
1/30. |
靶标
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功能
Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases. -
组织特异性
Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages. -
序列相似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11326 Human
- Entrez Gene: 278180 Mouse
- Omim: 300353 Human
- SwissProt: Q9Y279 Human
- Unigene: 8904 Human
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别名
- Complement receptor of the immunoglobulin superfamily antibody
- CRIg antibody
- Ig superfamily protein antibody
see all
图片
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling ab252933 with VSIG4 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000) dilution. Positive staining on Kupffer cells of mouse liver (PMID: 17016562) is observed. The nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .
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VSIG4 was immunoprecipitated from 0.35 mg of human placenta lysate with ab252933 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252933 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Human placenta lysate 10 μg (Input).
Lane 2: ab252933 IP in human placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252933 in human placenta lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular weight observed is consistent with what has been described in previous literature (PMID: 16009265, 17016562).
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of mouse liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
All lanes : Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 37,50 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 125 seconds.
The molecular weight observed is consistent with what has been described in previous literatures (PMID: 16009265, 17016562)
The expression profile observed is consistent with the literatures (PMID: 16009265, 17016562).
Negative control: Jurkat (PMID: 16009265).
This blot was developed using a higher sensitivity ECL substrate.
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Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution + Mouse liver tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 17016562, PMID: 17016562).
This blot was developed using a higher sensitivity ECL substrate.
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Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution + His-tagged human VSIG4 recombinant protein (aa 19-232) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking /diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab252933 被引用在 1 文献中.
- Liang L et al. Establishment and validation of a novel invasion-related gene signature for predicting the prognosis of ovarian cancer. Cancer Cell Int 22:118 (2022). PubMed: 35292033