重组Anti-VPS41抗体[EPR13268] (ab181078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13268] to VPS41
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-VPS41抗体[EPR13268]
参阅全部 VPS41 一抗 -
描述
兔单克隆抗体[EPR13268] to VPS41 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, human fetal brain, Mouse brain, RAW264.7 and Rat brain lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13268 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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说明 |
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WB
1/1000 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
靶标
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相关性
VPS41 is required for vacuolar assembly and vacuolar traffic. Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. VSP41 is the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. -
细胞定位
Cytoplasmic face of coated pits and vesicles. -
数据库链接
- Entrez Gene: 27072 Human
- Entrez Gene: 218035 Mouse
- Entrez Gene: 306991 Rat
- Omim: 605485 Human
- SwissProt: P49754 Human
- SwissProt: Q5KU39 Mouse
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别名
- HVPS41 antibody
- hVps41p antibody
- HVSP41 antibody
see all
图片
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All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human fetal brain lysates
Lane 3 : Mouse brain lysates
Lane 4 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa -
All lanes : Anti-VPS41 antibody [EPR13268] (ab181078) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 99 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab181078 被引用在 5 文献中.
- Wen QX et al. AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice. Traffic 24:20-33 (2023). PubMed: 36412210
- Gu Y et al. Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. EMBO J 38:e101994 (2019). PubMed: 31625181
- Marwaha R et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). PubMed: 28325809
- Sindhwani A et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). PubMed: 29084291
- McLelland GL et al. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol 214:275-91 (2016). PubMed: 27458136