Anti-VPS35抗体- C-terminal (ab226180)
Key features and details
- Rabbit polyclonal to VPS35 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-VPS35抗体- C-terminal
参阅全部 VPS35 一抗 -
描述
兔多克隆抗体to VPS35 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human VPS35 aa 746-796 (C terminal). The exact sequence is proprietary. (NP_060676.2).
Database link: Q96QK1 -
阳性对照
- WB: HeLa, HEK-293T, Jurkat, TCMK-I and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226180 was affinity purified using an epitope specific to VPS35 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226180于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 92 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
组织特异性
Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. -
序列相似性
Belongs to the VPS35 family. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: ID: 465139 Chimpanzee
- Entrez Gene: 521864 Cow
- Entrez Gene: 101926811 Cynomolgus monkey
- Entrez Gene: 101142245 Gorilla
- Entrez Gene: 100056512 Horse
- Entrez Gene: 100133770 Human
- Entrez Gene: 55737 Human
- Entrez Gene: 65114 Mouse
see all -
别名
- DKFZp434E1211 antibody
- DKFZp434P1672 antibody
- FLJ10752 antibody
see all
图片
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Western blot - Anti-VPS35 antibody - C-terminal (ab226180)All lanes : Anti-VPS35 antibody - C-terminal (ab226180) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-VPS35 antibody - C-terminal (ab226180)VPS35 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226180 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226180 at 0.4 µg/ml.
Lane 1: ab226180 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab226180 被引用在 3 文献中.
- Hong X et al. ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells. Sci Rep 12:9700 (2022). PubMed: 35690672
- Ding Y et al. Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread. Cells 10:N/A (2021). PubMed: 33800686
- Turcotte MA et al. Guanine Nucleotide-Binding Protein-Like 1 (GNL1) binds RNA G-quadruplex structures in genes associated with Parkinson's disease. RNA Biol 18:1339-1353 (2021). PubMed: 33305682