AB227861

Anti-VPS34 抗体 - N-terminal

Name: VPS34抗体- N-terminal (ab227861)| Abcam中文官网
Brand: Abcam Limited
SKU: AB227861
Price: 4890 CNY
Availability: InStock

Anti-VPS34 antibody - N-terminal

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(5 Publications)

Rabbit Polyclonal VPS34 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIK3C3.

查看别名

VPS34, PIK3C3, Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34

5 Images

Supplier Data

Immunoprecipitation - Anti-VPS34 antibody - N-terminal (AB227861)

VPS34 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab227861. Western blot was performed from the immunoprecipitate using ab227861. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : HeLa whole cell extract.

Lane 2 : Control IgG IP in HeLa whole cell extract.

Lane 3 : ab227861 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-VPS34 antibody - N-terminal (ab227861)

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

VPS34 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg

Lane 3:

IMR32 whole cell lysate at 30 µg

Lane 4:

SK-N-AS whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-VPS34 antibody - N-terminal (ab227861) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

VPS34 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Recombinant Fragment Protein within Human PIK3C3. The exact immunogen used to generate this antibody is proprietary information.

Q8NEB9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed : 14617358, PubMed : 33637724, PubMed : 7628435). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed : 32690950). Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).. (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.

See full target information PIK3C3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology :18774 PubMed38916106

2024

WITHDRAWN: SUMO-specific peptidase 3 mediates the SUMO3 modification of BECN1 to repress cell autophagy in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Pujia Huang,Wenbo Kang,Haibo Chen,Jing Tian

The EMBO journal 43:931-955 PubMed38360997

2024

VHL suppresses autophagy and tumor growth through PHD1-dependent Beclin1 hydroxylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Meisi Yan,Leiguang Ye,Qimin Zhou,Yuran Duan,Hongfei Jiang,Lei Wang,Yuan Ouyang,Huahe Zhang,Yuli Shen,Guimei Ji,Xiaohan Chen,Qi Tian,Liwei Xiao,Qingang Wu,Ying Meng,Guijun Liu,Leina Ma,Bo Lei,Zhimin Lu,Daqian Xu

American journal of cancer research 12:5552-5563 PubMed36628278

2022

Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxiu Xu,Mingjie Chen,Mingyu Hu,Hanlu Wang,Zhongqiang Zuo,Jianguo Wang,Zhiyin Xie

Nature communications 13:4146 PubMed35842429

2022

Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Wrobel,Sandra M Hill,Alvin Djajadikerta,Marian Fernandez-Estevez,Cansu Karabiyik,Avraham Ashkenazi,Victoria J Barratt,Eleanna Stamatakou,Anders Gunnarsson,Timothy Rasmusson,Eric W Miele,Nigel Beaton,Roland Bruderer,Yuehan Feng,Lukas Reiter,M Paola Castaldi,Rebecca Jarvis,Keith Tan,Roland W Bürli,David C Rubinsztein

Autophagy 18:309-319 PubMed33993848

2021

AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Na Li,Yi Zhang,Xu Wang,Miao Yin,Qun-Ying Lei

View all publications

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Anti-VPS34 抗体 - N-terminal

Anti-VPS34 antibody - N-terminal

Immunoprecipitation - Anti-VPS34 antibody - N-terminal (AB227861)

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)

Western blot - Anti-VPS34 antibody - N-terminal (AB227861)