重组Anti-VPS34抗体[EPR5301] (ab124905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5301] to VPS34
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-VPS34抗体[EPR5301]
参阅全部 VPS34 一抗 -
描述
兔单克隆抗体[EPR5301] to VPS34 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human VPS34 aa 800-900. The exact sequence is proprietary.
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阳性对照
- L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.27 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5301 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-VPS34 antibody [EPR5301] - BSA and Azide free (ab240006)
- APC Anti-VPS34 antibody [EPR5301] (ab319253)
- PE Anti-VPS34 antibody [EPR5301] (ab319401)
- Alexa Fluor® 488 Anti-VPS34 antibody [EPR5301] (ab319535)
- Alexa Fluor® 647 Anti-VPS34 antibody [EPR5301] (ab319648)
- Alexa Fluor® 594 Anti-VPS34 antibody [EPR5301] (ab319752)
- Alexa Fluor® 555 Anti-VPS34 antibody [EPR5301] (ab319894)
- Alexa Fluor® 750 Anti-VPS34 antibody [EPR5301] (ab320988)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124905于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
组织特异性
Ubiquitously expressed, with a highest expression in skeletal muscle. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
细胞定位
Midbody. - Information by UniProt
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数据库链接
- Entrez Gene: 5289 Human
- Entrez Gene: 225326 Mouse
- Entrez Gene: 65052 Rat
- Omim: 602609 Human
- SwissProt: Q8NEB9 Human
- SwissProt: Q6PF93 Mouse
- SwissProt: O88763 Rat
- Unigene: 464971 Human
see all -
别名
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
see all
图片
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All lanes : Anti-VPS34 antibody [EPR5301] (ab124905) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : SHSY-5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab124905 被引用在 15 文献中.
- Shi D et al. Autophagy is induced by swine acute diarrhea syndrome coronavirus through the cellular IRE1-JNK-Beclin 1 signaling pathway after an interaction of viral membrane-associated papain-like protease and GRP78. PLoS Pathog 19:e1011201 (2023). PubMed: 36888569
- Li L et al. Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3. Bioengineered 13:5613-5624 (2022). WB ; Human . PubMed: 35184640
- Gandhirajan A et al. SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure. Front Immunol 13:1079962 (2022). PubMed: 36865524
- Liu Y et al. TNF-a released from retinal Müller cells aggravates retinal pigment epithelium cell apoptosis by upregulating mitophagy during diabetic retinopathy. Biochem Biophys Res Commun 561:143-150 (2021). PubMed: 34023779
- Dai F et al. Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy. Front Pharmacol 12:705747 (2021). PubMed: 34483910