Anti-VPS29抗体(ab10160)
Key features and details
- Goat polyclonal to VPS29
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VPS29抗体
参阅全部 VPS29 一抗 -
描述
山羊多克隆抗体to VPS29 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog
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免疫原
Synthetic peptide: DDVKVERIEYKKP, corresponding to C terminal amino acids 170-182 of Human VPS29.
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阳性对照
- Human muscle and kidney extracts.
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常规说明
VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody说明
VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10160于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). -
组织特异性
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain. -
序列相似性
Belongs to the VPS29 family. -
细胞定位
Cytoplasm. Membrane. Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 477477 Dog
- Entrez Gene: 51699 Human
- Entrez Gene: 56433 Mouse
- Entrez Gene: 288666 Rat
- Omim: 606932 Human
- SwissProt: Q9UBQ0 Human
- SwissProt: Q9QZ88 Mouse
- SwissProt: B2RZ78 Rat
see all -
别名
- DC15 antibody
- DC7 antibody
- DKFZp564F0223 antibody
see all
图片
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Western blot - Anti-VPS29 antibody (ab10160)Anti-VPS29 antibody (ab10160) at 0.1 µg/ml + Human Spleen lysate (RIPA buffer, 35µg total protein per lane)
Predicted band size: 20 kDa
Observed band size: 20 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab10160 被引用在 10 文献中.
- Chen X et al. VPS35 and α-Synuclein fail to interact to modulate neurodegeneration in rodent models of Parkinson's disease. Mol Neurodegener 18:51 (2023). PubMed: 37542299
- Sargent D et al. Neuronal VPS35 deletion induces spinal cord motor neuron degeneration and early post-natal lethality. Brain Commun 3:fcab208 (2021). PubMed: 34704029
- Li JG et al. A pharmacological chaperone improves memory by reducing Aß and tau neuropathology in a mouse model with plaques and tangles. Mol Neurodegener 15:1 (2020). PubMed: 31964406
- Crawley-Snowdon H et al. Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Nat Commun 11:5031 (2020). PubMed: 33024112
- Rahman AA et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N. Neuroscience 441:33-45 (2020). PubMed: 32540366
- Chen X et al. Parkinson's disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration. Proc Natl Acad Sci U S A 116:5765-5774 (2019). PubMed: 30842285
- Li JG et al. Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35. Mol Psychiatry N/A:N/A (2019). PubMed: 30733594
- Williams ET et al. Parkin mediates the ubiquitination of VPS35 and modulates retromer-dependent endosomal sorting. Hum Mol Genet 27:3189-3205 (2018). PubMed: 29893854
- Singh V et al. Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane. J Cell Sci 131:N/A (2018). PubMed: 30030371
- Zavodszky E et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). WB ; Human . PubMed: 24819384