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AB249413

重组Anti-VPS28抗体[EPR9881(B)] - BSA and Azide free

Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal VPS28 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

查看别名

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS28 with ab167172 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling VPS28 with ab167172 at 1/100 dilution.

Western blot - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)
  • WB

Unknown

Western blot - Anti-VPS28 antibody [EPR9881(B)] - BSA and Azide free (AB249413)

This data was developed using ab167172, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-VPS28 antibody [EPR9881(B)] (<a href='/products/primary-antibodies/vps28-antibody-epr9881b-ab167172'>ab167172</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa

false

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9881(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

ab249413 is the carrier-free version of ab167172.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
See full target information VPS28
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搭配使用产品

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

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Anti-VPS37A/HCRP1 antibody

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  • 0
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Primary Antibodies

AB185548

Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

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Primary Antibodies

AB7780

Anti-Ubiquitin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody (AB7780)

  • 4
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