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使用敲除细胞株进行验证重组RabMAb

重组Anti-VPS26抗体[EPR13455(B)] - N-terminal (ab180172)

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Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
  • Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
  • Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13455(B)] to VPS26 - N-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-VPS26抗体[EPR13455(B)] - N-terminal
    参阅全部 VPS26 一抗

  • 描述

    兔单克隆抗体[EPR13455(B)] to VPS26 - N-terminal

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human VPS26 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: O75436

  • 阳性对照

    • WB: HepG2, HeLa and 293T cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180172于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).

  • 序列相似性

    Belongs to the VPS26 family.

  • 细胞定位

    Cytoplasm. Endosome membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • FLJ12930 antibody
    • HB 58 antibody
    • HB58 antibody
    • Hbeta 58 antibody
    • Hbeta 58 homolog antibody
    • Hbeta58 antibody
    • Hbeta58 homolog antibody
    • hVPS 26 antibody
    • hVPS26 antibody
    • MGC94168 antibody
    • PEP 8A antibody
    • PEP8A antibody
    • Vacuolar protein sorting 26 A antibody
    • Vacuolar protein sorting 26 antibody
    • Vacuolar protein sorting 26 homolog A antibody
    • Vacuolar protein sorting 26 homolog A S. pombe antibody
    • Vacuolar protein sorting 26 homolog A yeast antibody
    • Vacuolar protein sorting 26 yeast antibody
    • Vacuolar protein sorting 26 yeast homolog antibody
    • Vacuolar protein sorting 26A antibody
    • Vacuolar protein sorting associated protein 26A antibody
    • Vacuolar protein sorting-associated protein 26A antibody
    • Vesicle protein sorting 26 antibody
    • Vesicle protein sorting 26A antibody
    • VP26A_HUMAN antibody
    • VPS 26 antibody
    • VPS 26A antibody
    • VPS26A antibody
    see all

图片

  • Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
    Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180172 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab180172 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab180172 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
    Western blot - Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
    All lanes : Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)
    Anti-VPS26 antibody [EPR13455(B)] - N-terminal (ab180172)

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