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重组RabMAb

重组Anti-VPS18抗体[EPR13378] - N-terminal (ab178416)

Reviews (1) Submit a question References (4)
Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
  • Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
  • Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13378] to VPS18 - N-terminal
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-VPS18抗体[EPR13378] - N-terminal
    参阅全部 VPS18 一抗

  • 描述

    兔单克隆抗体[EPR13378] to VPS18 - N-terminal

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details
    不适用于: IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human VPS18 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P253

  • 阳性对照

    • HeLa, 293T and K562 cell lysates; 293T cells; K562 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR13378

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab178416于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
ICC/IF
1/100 - 1/250.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
ICC/IF
1/100 - 1/250.
应用说明
Is unsuitable for IHC-P or IP.

靶标

  • 功能

    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

  • 组织特异性

    Ubiquitous. Expression was highest in heart and low in lung.

  • 序列相似性

    Belongs to the VPS18 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.

  • 细胞定位

    Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
  • Information by UniProt

  • 数据库链接

    • 别名

      • hVPS18 antibody
      • PEP3 antibody
      • Vacuolar protein sorting 18 antibody
      • Vacuolar protein sorting protein 18 antibody
      • Vacuolar protein sorting-associated protein 18 homolog antibody
      • vps18 antibody
      • VPS18_HUMAN antibody
      see all

    图片

    • Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
      Western blot - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
      All lanes : Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 110 kDa
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
      Immunocytochemistry/ Immunofluorescence - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

      Immunofluorescent analysis of 293T cells labeling VPS18 with ab178416 at 1/100 (green) and DAPI staining (blue).

    • Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
      Flow Cytometry (Intracellular) - Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

      Intracellular flow cytometrical analysis of permeabilized K562 cells labeling VPS18 with ab178416 at 1/10 (red) or a rabbit IgG (negative) (green). 

       

       

    • Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)
      Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

    文献 (4)

    发表研究结果有使用 ab178416？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab178416 被引用在 4 文献中.

    • Zhang S  et al. C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy. Nat Commun 14:6360 (2023). PubMed: 37821429
    • Jongsma ML  et al. SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J 39:e102301 (2020). PubMed: 32080880
    • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). PubMed: 28325809
    • Sindhwani A  et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). PubMed: 29084291

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    Western blot abreview for Anti-VPS18 antibody [EPR13378] - N-terminal

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Neuro-2a cells (mouse neuroblastoma))
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Loading amount
    10 µg
    Specification
    Neuro-2a cells (mouse neuroblastoma)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Aug 05 2021

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