AB183334

Anti-VPAC2 抗体 [SP235]

Anti-VPAC2 antibody [SP235]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal VPAC2 antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

查看别名

VIP2R, VIPR2, Vasoactive intestinal polypeptide receptor 2, VIP-R-2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, VPAC2, PACAP type III receptor, PACAP-R-3, PACAP-R3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (AB183334)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (AB183334)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling VPAC2 using ab183334 at a 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (AB183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-VPAC2 antibody [SP235] (AB183334)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VPAC2 antibody [SP235] (AB183334)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling VPAC2 with purified ab183334 at 1/20 dilution (5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VPAC2 antibody [SP235] (AB183334)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling VPAC2 using ab183334 (green) compare to negative control of rabbit IgG (blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (AB183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (AB183334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

不同偶联物与剂型 (1)

Carrier free

Anti-VPAC2 antibody [SP235] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP235

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from TCS by protein A/G.

存储溶液

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.

See full target information VIPR2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:835 PubMed40999516

2025

Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daoliang Jiang,Xiaoli Xu,Wenjing Yang,Mengjia Tang,Yuping Zhang,Lingfeng Shi,Jiongyu Hu

The Journal of clinical investigation 135: PubMed40590229

2025

Mitofusin 2 controls mitochondrial and synaptic dynamics of suprachiasmatic VIP neurons and related circadian rhythms.

Applications

Unspecified application

Species

Unspecified reactive species

Milan Stoiljkovic,Jae Eun Song,Hee-Kyung Hong,Heiko Endle,Luis Varela,Jonatas Catarino,Xiao-Bing Gao,Zong-Wu Liu,Peter Sotonyi,Sabrina Diano,Jonathan Cedernaes,Joseph Bass,Tamas L Horvath

Frontiers in oncology 12:852358 PubMed36237322

2022

Vasoactive intestinal peptide-VIPR2 signaling regulates tumor cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Asano,Misa Yamasaka,Kairi Ozasa,Kotaro Sakamoto,Atsuko Hayata-Takano,Takanobu Nakazawa,Hitoshi Hashimoto,James A Waschek,Yukio Ago

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VPAC2 抗体 [SP235]