Anti-VP16 tag抗体(ab4808)
Key features and details
- Rabbit polyclonal to VP16 tag
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-VP16 tag抗体 -
描述
兔多克隆抗体to VP16 tag -
宿主
Rabbit -
特异性
This antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product. -
经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody recognises GAL4-VP16 recombinant fusion protein (30kDa) in Western blot.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Detects a band of approximately 30 kDa.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 30 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (ab4808)This image is courtesy of an Abreview submitted on 10 March 2006.
ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488® conjugated Donkey anti-Rabbit IgG.
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Western blot - Anti-VP16 tag antibody (ab4808)Lane 1 : Marker
Lanes 2-3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml
Lane 2 : GAL4-VP16 human recombinant E.coli
Lane 3 : GAL4-VP16 human recombinant E.coli
with VP16 tag peptide (ab13406) at 1 µg/ml
Secondary
Lanes 2-3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab4808 被引用在 27 文献中.
- Weber TA et al. γ-Secretase modulators show selectivity for γ-secretase-mediated amyloid precursor protein intramembrane processing. J Cell Mol Med 26:880-892 (2022). PubMed: 34931449
- Cho KF et al. A light-gated transcriptional recorder for detecting cell-cell contacts. Elife 11:N/A (2022). PubMed: 35311648
- Moulinier-Anzola J et al. TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants. Mol Plant 13:717-731 (2020). PubMed: 32087370
- Nakamichi N et al. Flowering time control in rice by introducing Arabidopsis clock-associated PSEUDO-RESPONSE REGULATOR 5. Biosci Biotechnol Biochem 84:970-979 (2020). PubMed: 31985350
- Rodrigue A et al. A global functional analysis of missense mutations reveals two major hotspots in the PALB2 tumor suppressor. Nucleic Acids Res 47:10662-10677 (2019). PubMed: 31586400