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Anti-Von Willebrand Factor抗体[F8/86] (ab778)

Reviews (3)Q&A (2)References (11)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)

    Key features and details

    • Mouse monoclonal [F8/86] to Von Willebrand Factor
    • Suitable for: IHC-P, IHC-Fr
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Von Willebrand Factor抗体[F8/86]
      参阅全部 Von Willebrand Factor 一抗

    • 描述

      小鼠单克隆抗体[F8/86] to Von Willebrand Factor

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-P, IHC-Frmore details

    • 种属反应性

      与反应: Human
      预测可用于: Rabbit

    • 免疫原

      Full length native protein (purified) corresponding to Human Von Willebrand Factor. Von Willebrand Factor isolated from human plasma.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab778于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (3)
    1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method. We suggest an incubation period of 60 minutes at room temperature.
    说明
    IHC-P
    1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.

    ABC method. We suggest an incubation period of 60 minutes at room temperature.

    靶标

    • 功能

      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

    • 组织特异性

      Plasma.

    • 疾病相关

      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.

    • 序列相似性

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.

    • 结构域

      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.

    • 翻译后修饰

      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.

    • 细胞定位

      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Coagulation factor VIII antibody
        • Coagulation factor VIII VWF antibody
        • F8VWF antibody
        • Factor VIII related antigen antibody
        • von Willebrand antigen 2 antibody
        • von Willebrand antigen II antibody
        • Von Willebrand disease antibody
        • Von Willebrand factor precursor antibody
        • VWD antibody
        • vWF antibody
        • VWF_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody [F8/86] (ab778)
        Formalin fixed paraffin embedded human tonsil tissue, staining von Willebrand factor with ab778 in immunohistochemical analysis

      文献 (11)

      发表研究结果有使用 ab778？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab778 被引用在 11 文献中.

      • Liu T  et al. MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14. Ann Transl Med 9:232 (2021). PubMed: 33708859
      • Wan X  et al. Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis. Stem Cell Res Ther 11:535 (2020). PubMed: 33308306
      • Zhang W  et al. Cell-free therapy based on adipose tissue stem cell-derived exosomes promotes wound healing via the PI3K/Akt signaling pathway. Exp Cell Res 370:333-342 (2018). PubMed: 29964051
      • Chen JH  et al. Protective effects of atorvastatin on cerebral vessel autoregulation in an experimental rabbit model of subarachnoid hemorrhage. Mol Med Rep 17:1651-1659 (2018). PubMed: 29257200
      • Wang Y  et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016). IHC-P ; Rabbit . PubMed: 27151366
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-5 of 5 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer
      Sample
      Rabbit Tissue sections (trachea)
      Specification
      trachea
      Permeabilization
      Yes - triton X-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Ms. Hee-Bok Kim

      Verified customer

      提交于 Jun 05 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Poor
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Brain)
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Proteinase K
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Dako Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Mark Butters

      Verified customer

      提交于 Aug 19 2022

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody [F8/86]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
      Antigen retrieval step
      None
      Sample
      Human Tissue sections (blood vessels)
      Specification
      blood vessels
      Permeabilization
      Yes - saponine
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 24 2014

      Question

      I've used your mouse monoclonal von willebrand Factor antibody (ab778)for IHC in my experiments. The data will be published. The edditors asked us to show the specificity of this antibody. Your data sheet showed 3 references. I checked them and found no one exactely used this antibody for IHC. Could you tell me where the image (a good staining)in the data sheet was cited from? Do you have the antigen for this antibody? Or could you tell me what company has this antigen? I will buy the antigen of this antobody to run the preabsorption experiment.

      Read More
      Answer

      Thank you for contacting us.

      The lab sent me the following information regarding your question:

      Based on the data Factor VIII reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels and shows finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes.

      Please let me know if this information is sufficient.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      I've used your mouse monoclonal von willebrand Factor antibody (ab778)for IHC in my experiments. The data will be published. The edditors asked us to show the specificity of this antibody. Your data sheet showed 3 references. I checked them and found no one exactely used this antibody for IHC. Could you tell me where the image (a good staining)in the data sheet was cited from? Do you have the antigen for this antibody? Or could you tell me what company has this antigen? I will buy the antigen of this antobody to run the preabsorption experiment.

      Read More
      Answer

      Thank you for contacting us.

      I have emailed the lab to check regarding the specificity testing, and I'll let you know what I find out.
      The IHC image on the datasheet is from the lab.

      The immunogen used to raise the antibody was Von Willebrand Factor isolated from human plasma. We carry vWF protein (ab88533 and ab88555) that can be used for blocking, although we have not tried that application with these 2 proteins.

      https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88533.html
      https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88555.html

      You can use our testing discount program to try either protein in a blocking experiment.

      For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

      The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
      This programapplies to ELISA kits and other kits we offer.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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