Anti-Von Willebrand Factor抗体(ab6994)
Key features and details
- Rabbit polyclonal to Von Willebrand Factor
- Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFl
- Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Von Willebrand Factor抗体
参阅全部 Von Willebrand Factor 一抗 -
描述
兔多克隆抗体to Von Willebrand Factor -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFlmore details -
种属反应性
与反应: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
不与反应: Chicken -
免疫原
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.
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阳性对照
- IHC-P: Human kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (10) |
Use at an assay dependent concentration.
|
| ICC/IF | (8) |
Use at an assay dependent concentration.
|
| IHC-Fr | (14) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| IHC-P | (22) |
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol.
Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary.
Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
|
| IHC-FoFr | (3) |
Use at an assay dependent concentration. PubMed: 19622235
|
| IHC-FrFl | (2) |
Use at an assay dependent concentration.
(see Abreview) |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19622235 |
|
IHC-FrFl
Use at an assay dependent concentration. (see Abreview) |
靶标
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功能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
组织特异性
Plasma. -
疾病相关
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻译后修饰
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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数据库链接
- Entrez Gene: 7450 Human
- Entrez Gene: 116669 Rat
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q62935 Rat
- Unigene: 440848 Human
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别名
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (457)
ab6994 被引用在 457 文献中.
- Abu-Shahba AG et al. Periosteal Flaps Enhance Prefabricated Engineered Bone Reparative Potential. J Dent Res 101:166-176 (2022). PubMed: 34514892
- Al-Maawi S et al. Covalent linkage of sulfated hyaluronan to the collagen scaffold Mucograft® enhances scaffold stability and reduces proinflammatory macrophage activation in vivo. Bioact Mater 8:420-434 (2022). PubMed: 34541411
- Chen Y et al. Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. Theranostics 12:910-928 (2022). PubMed: 34976220
- Yan J et al. Met-RANTES preserves the blood-brain barrier through inhibiting CCR1/SRC/Rac1 pathway after intracerebral hemorrhage in mice. Fluids Barriers CNS 19:7 (2022). PubMed: 35062973
- Hsieh LT et al. Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1β, are linked to fibrin formation in Buruli ulcer lesions. PLoS Pathog 18:e1010280 (2022). PubMed: 35100311