Anti-Von Hippel Lindau/VHL抗体[OTI1E1] - BSA and Azide free (ab273655)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394380-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-pancreas-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)")
Key features and details
- Mouse monoclonal [OTI1E1] to Von Hippel Lindau/VHL - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-Von Hippel Lindau/VHL抗体[OTI1E1] - BSA and Azide free
参阅全部 Von Hippel Lindau/VHL 一抗 -
描述
小鼠单克隆抗体[OTI1E1] to Von Hippel Lindau/VHL - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein aa 1 to the C-terminus. Produced in HEK-293T cells.
Database link: P40337 -
阳性对照
- WB: pCMV6-ENTRY VHL transfected HEK-293T cell lsyate. IHC-P: Human colon carcinoma, ovary adenocarcinoma, pancreas, endometrium adenocarcinoma, lung carcinoma, endometrium and bladder tissue.
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常规说明
ab273655 is the carrier free version of ab140989.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
OTI1E1 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273655于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/4000. Predicted molecular weight: 24 kDa.
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| IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/4000. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. -
组织特异性
Expressed in the adult and fetal brain and kidney. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Defects in VHL are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
Defects in VHL are the cause of von Hippel-Lindau disease (VHLD) [MIM:193300]. VHLD is a dominantly inherited familial cancer syndrome characterized by the development of retinal angiomatosis, cerebellar and spinal hemangioblastoma, renal cell carcinoma (RCC), phaeochromocytoma and pancreatic tumors. VHL type 1 is without pheochromocytoma, type 2 is with pheochromocytoma. VHL type 2 is further subdivided into types 2A (pheochromocytoma, retinal angioma, and hemangioblastomas without renal cell carcinoma and pancreatic cyst) and 2B (pheochromocytoma, retinal angioma, and hemangioblastomas with renal cell carcinoma and pancreatic cyst). VHL type 2C refers to patients with isolated pheochromocytoma without hemangioblastoma or renal cell carcinoma. The estimated incidence is 3/100000 births per year and penetrance is 97% by age 60 years.
Defects in VHL are the cause of erythrocytosis familial type 2 (ECYT2) [MIM:263400]; also called VHL-dependent polycythemia or Chuvash type polycythemia. ECYT2 is an autosomal recessive disorder characterized by an increase in serum red blood cell mass, hypersensitivity of erythroid progenitors to erythropoietin, increased erythropoietin serum levels, and normal oxygen affinity. Patients with ECYT2 carry a high risk for peripheral thrombosis and cerebrovascular events.
Defects in VHL are a cause of renal cell carcinoma (RCC) [MIM:144700]. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
结构域
The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV]. -
细胞定位
Cytoplasm. Membrane. Nucleus. Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated and Cytoplasm. Nucleus. Equally distributed between the nucleus and the cytoplasm but not membrane-associated. - Information by UniProt
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数据库链接
- Entrez Gene: 7428 Human
- Omim: 608537 Human
- SwissProt: P40337 Human
- Unigene: 517792 Human
- Unigene: 607789 Human
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别名
- Elongin binding protein antibody
- G7 protein antibody
- HRCA 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
Paraffin-embedded human pancreas tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
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![Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394373-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-transfected-hek293t.jpg)
Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)All lanes : Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (ab140989) at 1/4000 dilution
Lane 1 : HEK-293T cells were transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells were transfected with the pCMV6-ENTRY VHL cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDaCells were transfected with cDNA for 48 hours then lysed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394379-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-ovary-adenocarcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human ovary adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394378-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-lung-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human lung carcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394377-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-endometrium-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human endometrium tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394376-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-endometrium-adenocarcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human endometrium adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394375-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-bladder-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human bladder tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)](https://www.abcam.cn/ps/products/273/ab273655/Images/ab273655-394374-AntiVon-Hippel-Lindau-VHL-antibody-OTI1E1-immunohistochemistry-colon-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)Paraffin-embedded human colon adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab273655 尚未被引用在任何文献中。
