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AB242115

Anti-VMA21抗体

Anti-VMA21 antibody

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(1 Publication)

Rabbit Polyclonal VMA21 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VMA21 aa 1-50.

查看别名

MEAX, XMEA, VMA21, Vacuolar ATPase assembly integral membrane protein VMA21, Myopathy with excessive autophagy protein

2 Images
Western blot - Anti-VMA21 antibody (AB242115)
  • WB

Supplier Data

Western blot - Anti-VMA21 antibody (AB242115)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-VMA21 antibody (ab242115) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 11 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-VMA21 antibody (AB242115)
  • IP

Supplier Data

Immunoprecipitation - Anti-VMA21 antibody (AB242115)

VMA21 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242115 used for IP at 20 μl per reaction. For WB at 1/100 dilution.

Lane 1 : ab242115 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-VMA21 antibody (ab242115)

Predicted band size: 11 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human VMA21 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q3ZAQ7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibody was affinity purified using an epitope specific to VMA21 immobilized on solid support.
存储溶液
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VMA21 also known as Vacuolar H+-ATPase assembly factor VMA21 serves an important role in cellular mechanics. This protein with a molecular mass of approximately 9 kDa helps assemble the V-ATPase complex which is important for acidifying vacuoles. VMA21 is expressed in many tissues with higher levels observed in metabolically active tissues due to their need for efficient ion transport and pH regulation.
Biological function summary

VMA21 is an important factor in assembling the V-ATPase complex located in the membranes of vacuoles. The V-ATPase is a proton pump that regulates the pH within organelles by transporting hydrogen ions. VMA21 stabilizes the subunits of V-ATPase allowing its correct function in processes like protein degradation and recycling. This regulation is essential for maintaining a balance in the acidic environment necessary for various cellular processes.

Pathways

VMA21 interacts with the V-ATPase complex impacting the lysosomal acidification and nutrient sensing pathways. It connects with the mTORC1 signaling cascade by affecting lysosomal pH which in turn influences cell growth and metabolism. The protein also has indirect ties with SNARE proteins involved in vesicular transport exemplifying its role in broader biological activities.

VMA21 associates with X-linked Myopathy with Excessive Autophagy (XMEA). Mutations in VMA21 impair the V-ATPase activity leading to deficient lysosomal acidification and autophagy dysregulation. This impairment results in muscle disease characterized by increased autophagic vacuoles. VMA21 also affects other proteins involved in autophagy such as LC3 indicating its extensive role in muscle pathology and metabolic disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.
See full target information VMA21
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed33236988

2020

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmi E Miller-Vedam,Bastian Bräuning,Katerina D Popova,Nicole T Schirle Oakdale,Jessica L Bonnar,Jesuraj R Prabu,Elizabeth A Boydston,Natalia Sevillano,Matthew J Shurtleff,Robert M Stroud,Charles S Craik,Brenda A Schulman,Adam Frost,Jonathan S Weissman
View all publications
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