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重组RabMAb

重组Anti-Vitronectin/S-Protein抗体[EP873Y] (ab45139)

Submit a review Q&A (6)References (21)
Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
  • Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

概述

  • 产品名称

    Anti-Vitronectin/S-Protein抗体[EP873Y]
    参阅全部 Vitronectin/S-Protein 一抗

  • 描述

    兔单克隆抗体[EP873Y] to Vitronectin/S-Protein

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Vitronectin/S-Protein aa 450-550. The exact sequence is proprietary.

  • 阳性对照

    • IHC-P: Human liver tissue. WB: Human serum tissue lysate ICC/IF: HepG2 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP873Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab45139于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
说明
WB
1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

  • 组织特异性

    Plasma.

  • 序列相似性

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.

  • 结构域

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.

  • 翻译后修饰

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.

  • 细胞定位

    Secreted, extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution + Human serum tissue lysate at 20 µg with 5% NFDM/TBST

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

      Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)
      Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (21)

    发表研究结果有使用 ab45139？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab45139 被引用在 21 文献中.

    • Zheng Y  et al. Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma. J Cancer 14:114-128 (2023). PubMed: 36605486
    • Li J  et al. Urinary exosomal vitronectin predicts vesicoureteral reflux in patients with neurogenic bladders and spinal cord injuries. Exp Ther Med 23:65 (2022). PubMed: 34934436
    • Um SH  et al. Regulation of cell locomotion by nanosecond-laser-induced hydroxyapatite patterning. Bioact Mater 6:3608-3619 (2021). PubMed: 33869901
    • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
    • Brito VGB  et al. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production. PLoS One 16:e0247372 (2021). PubMed: 33661916
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    MSDS needed for
    ab3104
    ab18412
    ab45139
    ab68672

    Read More
    Answer

    Please find attached to this email the MSDS you requested for the following antibodies:
    ab3104
    ab18412
    ab45139
    ab68672

    A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few hours up to one day, delay that is necessary for the cache of the website to be regenerated.

    I hope these documents are useful to you. Do not hesitate to contact us again if you have further questions.

    I wish you good luck with your research work.

    Read More

    Question

    Dear,

    I put in this email all the reference for which I need a MSDS.

    ab45139
    ab3104
    ab68672
    ab45139
    ab18412

    Thanks in advance for your answer,

    Read More
    Answer

    Thank you for contacting us.

    I would like to inform you that we are currently dealing with your request.

    I will contact you again as soon as the requested documents are available.

    Thank you for your patience.

    Read More

    Question

    Hello Abcam staff,   When is the antibody arriving at NIH?

    Read More
    Answer

    Thank you for contacting Abcam.

    According to the details of our testing program we "offer a discount equal to one free antibody of your choice off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview."

    I have not seen any order from NIH for this product. Please be sure to contact your purchase agent to be sure that this product has been ordered. If it has been ordered we may track this product if you could supply either the Abcam confirmation number for the order or the PO number used to order ab45139.



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Question

    Thanks for the offer. Please send the sample: ab45139.



    We would like to test this antibody for western blotting and immunohistochemistry in mice eye.

    Read More
    Answer

    DISCOUNT CODE: ***********
    Expiration date: ************

    I am very pleased to hear you would like to accept our offer and test ab45139 in IHC-Frozen tissue.This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-Frozen tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Testing ab45139 in IHC-Fr tissue.

    Read More
    Answer

    Thank you very much for your interest in ab45139.

    To our knowledge, ab45139 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab45139 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab45139 so please wait for my reply before ordering.

    2. Purchase ab45139 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab45139 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Question

    ab may not be working in IHC; she tried both IHC-P and IHC-Fr of mouse liver sections; used 1:50, 1:100, 1:200 with pH6 heat retrieval for IHC-P; in all assays there was no staining; ; offered to try it one more time with frozen sections and will call back with results. Customer has called back and results with 1:10 dilution on IHC-Fr still negative.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62769.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

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