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重组RabMAb

重组Anti-Vitronectin/S-Protein抗体[EP781Y] (ab46808)

Submit a review Submit a question References (4)
Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
  • Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
  • Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP781Y] to Vitronectin/S-Protein
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Vitronectin/S-Protein抗体[EP781Y]
    参阅全部 Vitronectin/S-Protein 一抗

  • 描述

    兔单克隆抗体[EP781Y] to Vitronectin/S-Protein

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Vitronectin/S-Protein aa 100-200. The exact sequence is proprietary.

  • 阳性对照

    • WB: Human serum IHC-P: Human lung tissue. IP: Human serum

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.

  • 克隆

    单克隆

  • 克隆编号

    EP781Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab46808于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa).
IP
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

  • 组织特异性

    Plasma.

  • 序列相似性

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.

  • 结构域

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.

  • 翻译后修饰

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.

  • 细胞定位

    Secreted, extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
      Western blot - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
      Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808) at 1/20000 dilution + Human serum at 10 µg

      Secondary
      Goat anti-rabbit, HRP labelled. at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?
      Additional bands at: 54 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

      This image shows human lung tissue treated with ab46808 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
      Immunoprecipitation - Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

      Purified ab46808 at 1/50 dilution (2µg) immunoprecipitating Vitronectin/S-Protein in Human serum.
      Lane 1 (input): Human serum 10µg
      Lane 2 (+): ab46808 + Human serum.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab46808 in Human serum.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 65-75 kDa

    • Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)
      Anti-Vitronectin/S-Protein antibody [EP781Y] (ab46808)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab46808？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab46808 被引用在 4 文献中.

    • Ma J & Kan Z Circular RNA circ_0008274 enhances the malignant progression of papillary thyroid carcinoma via modulating solute carrier family 7 member 11 by sponging miR-154-3p. Endocr J 68:543-552 (2021). PubMed: 33473055
    • Miura K & Yamashita K Mechanical Weakness of Thoracic Aorta Related to Aging or Dissection Predicted by Speed of Sound with Collagenase. Ultrasound Med Biol 45:3102-3115 (2019). PubMed: 31537389
    • Shi K  et al. Vitronectin significantly influences prognosis in osteosarcoma. Int J Clin Exp Pathol 8:11364-71 (2015). PubMed: 26617861
    • Shen J  et al. Increased expression levels of vitronectin in the maternal-fetal interface of placenta in early-onset severe preeclampsia. Mol Med Rep 7:53-8 (2013). PubMed: 23124223

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