Anti-Vitronectin/S-Protein抗体(ab272865)
Key features and details
- Rabbit polyclonal to Vitronectin/S-Protein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Vitronectin/S-Protein抗体
参阅全部 Vitronectin/S-Protein 一抗 -
描述
兔多克隆抗体to Vitronectin/S-Protein -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human Vitronectin/S-Protein. The exact sequence is proprietary. Center region.
Database link: P04004 -
阳性对照
- WB: Mouse brain tissue extract. HepG2 conditioned medium. IHC-P: Human colon and lung carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272865于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
组织特异性
Plasma. -
序列相似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
结构域
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻译后修饰
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
细胞定位
Secreted, extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 7448 Human
- Entrez Gene: 22370 Mouse
- Omim: 193190 Human
- SwissProt: P04004 Human
- SwissProt: P29788 Mouse
- Unigene: 2257 Human
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别名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
图片
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Western blot - Anti-Vitronectin/S-Protein antibody (ab272865)Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab272865)Paraffin-embedded human colon carcinoma tissue stained for Vitronectin/S protein using ab272865 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab272865)Paraffin-embedded human lung carcinoma tissue stained for Vitronectin/S protein using ab272865 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-Vitronectin/S-Protein antibody (ab272865)All lanes : Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 conditioned medium
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272865 尚未被引用在任何文献中。