Anti-Vitronectin/S-Protein 抗体 [EP873Y]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Vitronectin/S-Protein antibody [EP873Y] (AB45139)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Vitronectin/S-Protein with purified ab45139 at 1/100 dilution (7 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.11 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody [EP873Y] (AB45139)

ab45139 at a 1/100 dilution staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (AB45139)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution

All lanes:

Human serum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 75 kDa

false

Exposure time: 30s